ArticleThe Journal of physiology2017
In vitro model to study the effects of matrix stiffening on Ca
Article in The Journal of physiology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 27 citations in OpenAlex.
- Engineering Thermoresponsive Biointerfaces Using Graft-To Strategies: Connecting Polymer Architecture to Temperature-Dependent Surface Wettability.Macromolecules · 2026Article
- Optical control of the βiScience · 2022Article
- Extracellular stiffness induces contractile dysfunction in adult cardiomyocytes via cell-autonomous and microtubule-dependent mechanisms.Basic research in cardiology · 2022Article
- The harder the climb the better the view: The impact of substrate stiffness on cardiomyocyte fate.Journal of molecular and cellular cardiology · 2022Review
- Conventional rigid 2D substrates cause complex contractile signals in monolayers of human induced pluripotent stem cell-derived cardiomyocytes.The Journal of physiology · 2022Article
- Cooperation between myofibril growth and costamere maturation in human cardiomyocytes.Frontiers in bioengineering and biotechnology · 2022Article
- Use of Proximity Labeling in Cardiovascular Research.JACC. Basic to translational science · 2021Review
- Substrate Stiffness Influences Structural and Functional Remodeling in Induced Pluripotent Stem Cell-Derived Cardiomyocytes.Frontiers in physiology · 2021Article
- Left ventricular dysfunction in heart failure with preserved ejection fraction-molecular mechanisms and impact on right ventricular function.Cardiovascular diagnosis and therapy · 2020Review
- Decellularized Extracellular Matrices and Cardiac Differentiation: Study on Human Amniotic Fluid-Stem Cells.International journal of molecular sciences · 2020Article
- Myocardial slices come to age: an intermediate complexity in vitro cardiac model for translational research.Cardiovascular research · 2020Review
- Nitric Oxide and Mechano-Electrical Transduction in Cardiomyocytes.Frontiers in physiology · 2020Review
- Large-Scale Contractility Measurements Reveal Large Atrioventricular and Subtle Interventricular Differences in Cultured Unloaded Rat Cardiomyocytes.Frontiers in physiology · 2020Article
- Cardiac Microvascular Endothelial Enhancement of Cardiomyocyte Function Is Impaired by Inflammation and Restored by Empagliflozin.JACC. Basic to translational science · 2019Article
- End-diastolic force pre-activates cardiomyocytes and determines contractile force: role of titin and calcium.The Journal of physiology · 2019Article
- Extracellular Matrix From Hypertrophic Myocardium Provokes Impaired Twitch Dynamics in Healthy Cardiomyocytes.JACC. Basic to translational science · 2019Article
- Costameres, dense plaques and podosomes: the cell matrix adhesions in cardiovascular mechanosensing.Journal of muscle research and cell motility · 2019Review
- Modeling cardiac complexity: Advancements in myocardial models and analytical techniques for physiological investigation and therapeutic developmentAPL bioengineering · 2019Review
- Isolation of Atrial Cardiomyocytes from a Rat Model of Metabolic Syndrome-related Heart Failure with Preserved Ejection Fraction.Journal of visualized experiments : JoVE · 2018Article
- Extracellular matrix stiffness affects contractility in adult rat cardiomyocytes: implications for dynamic nitric oxide signalling and calcium handling.The Journal of physiology · 2017Article
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
key pointsThis paper describes a novel model that allows exploration of matrix-induced cardiomyocyte adaptations independent of the passive effect of matrix rigidity on cardiomyocyte function. Detachment of adult cardiomyocytes from the matrix enables the study of matrix effects on cell shortening, Ca ABSTRACT: Extracellular matrix (ECM) stiffening is a key element of cardiac disease. Increased rigidity of the ECM passively inhibits cardiac contraction, but if and how matrix stiffening also actively alters cardiomyocyte contractility is incompletely understood. In vitro models designed to study cardiomyocyte-matrix interaction lack the possibility to separate passive inhibition by a stiff matrix from active matrix-induced alterations of cardiomyocyte properties. Here we introduce a novel experimental model that allows exploration of cardiomyocyte functional alterations in response to matrix stiffening. Adult rat cardiomyocytes were cultured for 24 h on matrices of tuneable stiffness representing the healthy and the diseased heart and detached from their matrix before functional measurements. We demonstrate that matrix stiffening, independent of passive inhibition, reduces cell shortening and Ca
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.