ArticleNature communications2017
Control of type III protein secretion using a minimal genetic system.
Article in Nature communications, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
33 citing papers in PubMed.
- Quantitative Dissection of Agrobacterium Virulence to Generate a Synthetic Ti Plasmid.ACS synthetic biology · 2026Article
- Tumor-specific cytokine therapy mediated by engineered Salmonella with a synthetic protein delivery system.Scientific reports · 2026Article
- Medium dependent factors govern the functionality of engineered type III secretion systems.Journal of biological engineering · 2025Article
- "A high throughput flow cytometry assay for quantifying type 3 secretion system assembly inbioRxiv : the preprint server for biology · 2025Article
- A high throughput assay for measuring secreted protein based on a de novo fluorescent reporter reveals regulatory and structural insights in Salmonella type three secretion system.Protein science : a publication of the Protein Society · 2025Article
- The design and engineering of synthetic genomes.Nature reviews. Genetics · 2025Review
- Engineering Agrobacterium for improved plant transformation.The Plant journal : for cell and molecular biology · 2025Review
- Evaluation of theFrontiers in bioengineering and biotechnology · 2025Article
- Combinatorial leaky probiotic for anticancer immunopotentiation and tumor eradication.Cell reports. Medicine · 2024Article
- Cytosolic delivery of monobodies using the bacterial type III secretion system inhibits oncogenic BCR: ABL1 signaling.Cell communication and signaling : CCS · 2024Article
- Article
- An Improved Method for Eliminating or Creating Intragenic Bacterial Promoters.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Construction of a constitutively active type III secretion system for heterologous protein secretion.Applied microbiology and biotechnology · 2023Article
- Overlapping genes in natural and engineered genomes.Nature reviews. Genetics · 2022Review
- Bacterial two-component systems as sensors for synthetic biology applications.Current opinion in systems biology · 2021Article
- The Type III Effectome of the SymbioticBiomolecules · 2021Article
- TheMicroorganisms · 2021Review
- An optimized growth medium for increased recombinant protein secretion titer via the type III secretion system.Microbial cell factories · 2021Article
- LITESEC-T3SS - Light-controlled protein delivery into eukaryotic cells with high spatial and temporal resolution.Nature communications · 2020Article
- A highly conserved complete accessory Escherichia coli type III secretion system 2 is widespread in bloodstream isolates of the ST69 lineage.Scientific reports · 2020Article
Corrections and comments
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Authors and funding
10 authors.
Funding
Abstract
Gram-negative bacteria secrete proteins using a type III secretion system (T3SS), which functions as a needle-like molecular machine. The many proteins involved in T3SS construction are tightly regulated due to its role in pathogenesis and motility. Here, starting with the 35 kb Salmonella pathogenicity island 1 (SPI-1), we eliminated internal regulation and simplified the genetics by removing or recoding genes, scrambling gene order and replacing all non-coding DNA with synthetic genetic parts. This process results in a 16 kb cluster that shares no sequence identity, regulation or organizational principles with SPI-1. Building this simplified system led to the discovery of essential roles for an internal start site (SpaO) and small RNA (InvR). Further, it can be controlled using synthetic regulatory circuits, including under SPI-1 repressing conditions. This work reveals an incredible post-transcriptional robustness in T3SS assembly and aids its control as a tool in biotechnology.
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Registered trials
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