ArticleMolecular vision2017
A dot hybridization assay for the diagnosis of bacterial keratitis.
Article in Molecular vision, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 13 citations in OpenAlex.
- Oxford Nanopore Technology-Based Identification of anMicroorganisms · 2024Article
- Monitoring the transition from corneal ulceration to healed scar using a Scheimpflug tomography-based densitometry.Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie · 2024Article
- Deep Learning Approach in Image Diagnosis ofDiagnostics (Basel, Switzerland) · 2022Article
- Diagnosing Fungal Keratitis and Simultaneously IdentifyingJournal of fungi (Basel, Switzerland) · 2022Article
- Diagnostic armamentarium of infectious keratitis: A comprehensive review.The ocular surface · 2022Review
- Comparisons of deep learning algorithms for diagnosing bacterial keratitis via external eye photographs.Scientific reports · 2021Article
- A Genomic Approach to Investigating Ocular Surface Microorganisms: Monitoring Core Microbiota on Eyelid Margin with a Dot hybridization Assay.International journal of molecular sciences · 2020Article
- A deep learning approach in diagnosing fungal keratitis based on corneal photographs.Scientific reports · 2020Article
- Comparison of fungal and bacterial keratitis between tropical and subtropical Taiwan: a prospective cohort study.Annals of clinical microbiology and antimicrobials · 2020Article
- A DNA dot hybridization model for molecular diagnosis of parasitic keratitis.Molecular vision · 2017Article
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeTo evaluate a bacterial dot hybridization (BDH) assay for the diagnosis of bacterial keratitis (BK).
methodsSixty-one qualified corneal scrapings from 61 patients with suspected microbial keratitis were collected consecutively and prospectively. Among the 61 patients, 16 cases were BK and 45 cases were non-BK, including fungal keratitis, viral keratitis, parasitic keratitis, and non-microbial keratitis. Molecular diagnosis of BK in these corneal scrapes was performed using the BDH assay with three universal bacterial probes (PB1, PB2, and PB3) and three genus-specific probes (Aci, Klb, and Psu) to detect
resultsThe standardized intensities for the three universal probes differed statistically significantly between the BK group and the non-BK group. Based on the ROC curves, the sensitivities of PB1, PB2, and PB3 were 81.3%, 81.3%, and 93.8%, and the specificities were 71.1%, 88.9%, and 91.1%, respectively. The sensitivity and specificity of the Psu probe were 92% and 100%, respectively, while those of the Aci and Klb probes could not be estimated because there were no BK cases caused by
conclusionsThe BDH assay is an effective molecular approach to improve the diagnosis of BK. Because the bias from bacterial contamination on the ocular surface can be minimized with signal standardization, the assay has the potential to be adopted for routine clinical practice.
Indexed as
Identifiers
28484310PMC5410166W2619642023What OpenQuestion holds
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