ArticleParasites & vectors2017
Caspase-1 participates in apoptosis of salivary glands in Rhipicephalus haemaphysaloides.
Article in Parasites & vectors, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 26 citations in OpenAlex.
- Blood feeding triggers the terminal differentiation of precursor cells in tick salivary glands.Science advances · 2026Article
- Ferroptosis as a novel form of regulated cell death: Implications in the pathogenesis, oncometabolism and treatment of human cancer.Genes & diseases · 2022Review
- Potential Mechanisms of Transmission of Tick-Borne Viruses at the Virus-Tick Interface.Frontiers in microbiology · 2022Review
- Apoptosis and Autophagy: Current Understanding in Tick-Pathogen Interactions.Frontiers in cellular and infection microbiology · 2022Review
- Identification of the Bcl-2 and Bax homologs from Rhipicephalus haemaphysaloides and their function in the degeneration of tick salivary glands.Parasites & vectors · 2021Article
- Engorgement of Rhipicephalus haemaphysaloides ticks blocked by silencing a protein inhibitor of apoptosis.Experimental & applied acarology · 2021Article
- Borrelia burgdorferi infection modifies protein content in saliva of Ixodes scapularis nymphs.BMC genomics · 2021Article
- ATG5 is instrumental in the transition from autophagy to apoptosis during the degeneration of tick salivary glands.PLoS neglected tropical diseases · 2021Article
- Initiator and executioner caspases in salivary gland apoptosis of Rhipicephalus haemaphysaloides.Parasites & vectors · 2020Article
- Changes in Protein Phosphorylation during Salivary Gland Degeneration in Haemaphysalis longicornis.The Korean journal of parasitology · 2020Article
- Immunomodulatory effects of Rhipicephalus haemaphysaloides serpin RHS2 on host immune responses.Parasites & vectors · 2019Article
- Article
- Transcriptome and Proteome Response ofFrontiers in physiology · 2019Article
- Comprehensive Analysis of the Global Protein Changes That Occur During Salivary Gland Degeneration in Female Ixodid TicksFrontiers in physiology · 2018Article
- Relative transcription of autophagy-related genes in Amblyomma sculptum and Rhipicephalus microplus ticks.Experimental & applied acarology · 2017Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundTicks are among the most harmful vectors worldwide. Their salivary glands play essential roles in blood-feeding and pathogen transmission and undergo apoptosis after feeding. Although it was previously reported that salivary degeneration in ixodid ticks is in response to hormonal stimulation, questions still exist with the underlying mechanisms of salivary gland apoptosis.
methodsSalivary glands of Rhipicephalus haemaphysaloides were collected from 1 to 7 days after attachment to the host. TUNEL and Annexin V assays were used to check apoptosis during this time. To confirm the role of caspase-1, RNA interference was used to silence its expression, and the dynamic changes of associated cysteine proteases were also shown by quantitative real time PCR and western blot, while TUNEL and Annexin V assays were used to confirm apoptosis.
resultsIn the present study, apoptosis of salivary glands in R. haemaphysaloides occurred 3 or 4 days after attachment to the host as determined by TUNEL and Annexin V assays. The expression of caspase-1 increased at 5-7 days. When the latter was silenced by RNA interference, apoptosis in the salivary glands was delayed. While there seemed to be another form of cell death in salivary glands of ticks, such occurrence may be caused by compensatory autophagy which involved autophagy-related gene 4D.
conclusionsThis study describes the apoptosis of salivary glands in R. haemaphysaloides and the dynamic changes in cysteine proteases in this activity. Cysteine proteases were involved in this process, especially caspase-1. Caspase-1 participated in the apoptosis of salivary glands.
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