ArticleBMC veterinary research2017
A robust PCR for the differentiation of potential virulent strains of Haemophilus parasuis.
Article in BMC veterinary research, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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32 citing papers in PubMed.
- Exploring the pangenome of Mycoplasma hyorhinis in search of potential virulence markers.Scientific reports · 2025Article
- Development and Application of Fluorescent and Lateral Flow Dipstick Recombinase-Aided Amplification for Rapid Detection ofVeterinary sciences · 2025Article
- Pathotyping and molecular serotyping of clinical isolates of Glaesserella parasuis in Taiwan.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2025Article
- Pig nasal and rectal microbiotas are involved in the antibody response to Glaesserella parasuis.Scientific reports · 2025Article
- Genomic insights into diversity, antimicrobial resistance and virulence ofFrontiers in microbiology · 2025Article
- Biofilm formation in Streptococcus suis: in vitro impact of serovars and assessment of coinfections with other porcine respiratory disease complex bacterial pathogens.Veterinary research · 2024Article
- Transcriptome analysis reveals a new virulence-associated trimeric autotransporter responsible for Glaesserella parasuis autoagglutination.Veterinary research · 2024Article
- Genomic insight into the diversity ofmSphere · 2024Article
- Virulence and Antimicrobial Resistance Characterization ofAntibiotics (Basel, Switzerland) · 2024Article
- Intensive antibiotic treatment of sows with parenteral crystalline ceftiofur and tulathromycin alters the composition of the nasal microbiota of their offspring.Veterinary research · 2023Article
- Ceftiofur treatment of sows results in long-term alterations in the nasal microbiota of the offspring that can be ameliorated by inoculation of nasal colonizers.Animal microbiome · 2023Article
- Molecular characterization of Glaesserella parasuis strains circulating in North American swine production systems.BMC veterinary research · 2023Article
- Immune responses following neonatal vaccination with conserved F4 fragment of VtaA proteins from virulentVaccine: X · 2023Article
- Development of a new multiplex quantitative PCR for the detection of Glaesserella parasuis, Mycoplasma hyorhinis, and Mycoplasma hyosynoviae.MicrobiologyOpen · 2023Article
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- Molecular Characterization and Phylogenetic Analysis ofPathogens (Basel, Switzerland) · 2023Article
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- TbpBPathogens (Basel, Switzerland) · 2022Article
- Identification ofPathogens (Basel, Switzerland) · 2022Article
- Temporal Patterns of Phenotypic Antimicrobial Resistance and Coinfecting Pathogens inPathogens (Basel, Switzerland) · 2022Article
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundHaemophilus parasuis is the etiological agent of Glässer's disease in swine. H. parasuis comprises strains with heterogeneous virulence capacity, from non-virulent to highly virulent. Determination of the pathogenic potential of the strains is important for diagnosis and disease control. The virulence-associated trimeric autotransporters (vtaA) genes have been used to predict H. parasuis virulence by PCR amplification of their translocator domains. Here, we report a new and improved PCR designed to detect a different domain of the vtaA genes, the leader sequence (LS) as a diagnostic tool to predict virulence.
methodsA collection of 360 H. parasuis strains was tested by PCR with LS specific primers. Results of the PCR were compared with the clinical origin of the strains and, for a subset of strains, with their phagocytosis and serum resistance using a Chi-square test.
resultsLS-PCR was specific to H. parasuis, and allowed the differential detection of the leader sequences found in clinical and non-clinical isolates. Significant correlation was observed between the results of the LS-PCR and the clinical origin (organ of isolation) of the strains, as well as with their phagocytosis and serum susceptibility, indicating that this PCR is a good predictor of the virulence of the strains. In addition, this new PCR showed a full correlation with the previously validated PCR based on the translocator domain. LS-PCR could be performed in a wide range of annealing temperatures without losing specificity.
conclusionThis newly described PCR based on the leader sequence of the vtaA genes, LS-PCR, is a robust test for the prediction of the virulence potential of H. parasuis strains.
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