ArticleNucleic acids research2017
A non-canonical site reveals the cooperative mechanisms of microRNA-mediated silencing.
Article in Nucleic acids research, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 67 citations in OpenAlex.
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- MicroRNA-phytochemical interactome targeting thioredoxin-interacting protein: a narrative review at the nexus of RNA therapeutics, cancer biology, and metabolic regulation.Frontiers in oncology · 2026Review
- Dual regulatory role of hsa-miR-122b-5p in chikungunya virus infection via interaction with CHIKV 3'-UTR and HDAC4 modulation.Journal of virology · 2025Article
- Nuclear Argonaute:miRNA complexes recognize target sequences within chromatin-associated RNA and silence gene expression.Nucleic acids research · 2025Article
- LncRNA NRAD1 regulates the triple-negative breast cancer transcriptome by miRNA biogenesis, localization, and predominately non-ceRNA interactions.Scientific reports · 2025Article
- It is Time to Revisit miRNA Therapeutics.Nucleic acid therapeutics · 2025Review
- Insight into the Role of the miR-584 Family in Human Cancers.International journal of molecular sciences · 2024Review
- The miRNA-target interactions: An underestimated intricacy.Nucleic acids research · 2024Review
- Recent advances in understanding microRNA function and regulation in C. elegans.Seminars in cell & developmental biology · 2024Review
- Outside the limit: questioning the distance restrictions for cooperative miRNA binding sites.Cellular & molecular biology letters · 2023Article
- The developmentally timed decay of an essential microRNA family is seed-sequence dependent.Cell reports · 2022Article
- Reexamining assumptions about miRNA-guided gene silencing.Nucleic acids research · 2022Review
- A nuclear function for an oncogenic microRNA as a modulator of snRNA and splicing.Molecular cancer · 2022Article
- Sequence determinants as key regulators in gene expression of T cells.Immunological reviews · 2021Review
- Article
- Systemic CLIP-seq analysis and game theory approach to model microRNA mode of binding.Nucleic acids research · 2021Article
- microRNA-mediated translation repression through GYF-1 and IFE-4 in C. elegans development.Nucleic acids research · 2021Article
- miRNA interplay: mechanisms and consequences in cancer.Disease models & mechanisms · 2021Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Although strong evidence supports the importance of their cooperative interactions, microRNA (miRNA)-binding sites are still largely investigated as functionally independent regulatory units. Here, a survey of alternative 3΄UTR isoforms implicates a non-canonical seedless site in cooperative miRNA-mediated silencing. While required for target mRNA deadenylation and silencing, this site is not sufficient on its own to physically recruit miRISC. Instead, it relies on facilitating interactions with a nearby canonical seed-pairing site to recruit the Argonaute complexes. We further show that cooperation between miRNA target sites is necessary for silencing in vivo in the C. elegans embryo, and for the recruitment of the Ccr4-Not effector complex. Using a structural model of cooperating miRISCs, we identified allosteric determinants of cooperative miRNA-mediated silencing that are required for both embryonic and larval miRNA functions. Our results delineate multiple cooperative mechanisms in miRNA-mediated silencing and further support the consideration of target site cooperation as a fundamental characteristic of miRNA function.
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