ArticleBiotechnology and bioengineering2017
Transgenic SCs expressing GDNF-IRES-DsRed impair nerve regeneration within acellular nerve allografts.
Article in Biotechnology and bioengineering, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 20 citations in OpenAlex.
- Evaluation of a GDNF-eluting nanofibrous PCL conduit in a mouse model of peripheral nerve injury.RSC advances · 2026Article
- Induced Pseudomembrane Enrichment in Long Nerve Allograft Reconstruction.Muscle & nerve · 2025Article
- The GDNF-gel/HA-Mg conduit promotes the repair of peripheral nerve defects by regulating PPAR-γ/RhoA/ROCK signaling pathway.iScience · 2024Article
- MSCs overexpressing GDNF restores brain structure and neurological function in rats with intracerebral hemorrhage.Molecular biomedicine · 2023Article
- Pleiotrophin-Neuregulin1 promote axon regeneration and sorting in conduit repair of critical nerve gap injuries.Research square · 2023Article
- Repairing whole facial nerve defects with xenogeneic acellular nerve grafts in rhesus monkeys.Neural regeneration research · 2022Article
- GDNF to the rescue: GDNF delivery effects on motor neurons and nerves, and muscle re-innervation after peripheral nerve injuries.Neural regeneration research · 2022Review
- Designer, injectable gels to prevent transplanted Schwann cell loss during spinal cord injury therapy.Science advances · 2020Article
- GDNF Gene Therapy to Repair the Injured Peripheral Nerve.Frontiers in bioengineering and biotechnology · 2020Review
- Imaging in the repair of peripheral nerve injury.Nanomedicine (London, England) · 2019Article
- A microfluidic platform to study the effects of GDNF on neuronal axon entrapment.Journal of neuroscience methods · 2018Article
- An update-tissue engineered nerve grafts for the repair of peripheral nerve injuries.Neural regeneration research · 2018Article
- GDNF enhances human blood-nerve barrier function in vitro via MAPK signaling pathways.Tissue barriers · 2018Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Providing temporally regulated glial cell line-derived neurotrophic factor (GDNF) to injured nerve can promote robust axon regeneration. However, it is poorly understood why providing highly elevated levels of GDNF to nerve can lead to axon entrapment in the zone containing elevated GDNF. This limited understanding represents an obstacle to the translation of GDNF therapies to treat nerve injuries clinically. Here, we investigated how transgenic Schwann cells (SCs) overexpressing GDNF-IRES-DsRed impact nerve regeneration. Cultured primary SCs were transduced with lentiviruses (GDNF-overexpressing transgenic SCs), one of which provides the capability to express high levels of GDNF and regulate temporal GDNF expression. These SC groups were transplanted into acellular nerve allografts (ANAs) bridging a 14 mm rat sciatic nerve defect. GDNF-overexpressing transgenic SCs expressing GDNF for as little as 1 week decreased axon regeneration across ANAs and caused extensive extracellular matrix (ECM) remodeling. To determine whether additional gene expression changes beyond GDNF transgene expression occurred in GDNF-overexpressing transgenic SCs, microarray analysis of GDNF-overexpressing transgenic SCs compared to untreated SCs was performed. Microarray analysis revealed a set of common genes regulated in transgenic SC groups expressing high levels of GDNF compared to untreated SCs. A co-culture model of GDNF-overexpressing transgenic SCs with fibroblasts (FBs) revealed differential FB ECM-related gene expression compared to untreated SCs. These data suggest a component of axon entrapment is independent of GDNF's impact on axons. Biotechnol. Bioeng. 2017;114: 2121-2130. © 2017 Wiley Periodicals, Inc.
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Registered trials
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