ArticleACS nano2017
Selective Photomechanical Detachment and Retrieval of Divided Sister Cells from Enclosed Microfluidics for Downstream Analyses.
Article in ACS nano, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
10 citing papers in PubMed, 27 citations in OpenAlex.
- Advances in Single-Cell Techniques for Linking Phenotypes to Genotypes.Cancer heterogeneity and plasticity · 2024Article
- Recent Advancements in Ultrasound Transducer: From Material Strategies to Biomedical Applications.BME frontiers · 2022Review
- Dynamic acoustic focusing in photoacoustic transmitter.Photoacoustics · 2021Article
- Differential detachment of intact and viable cells of different sizes using laser-induced microbubbles.Biomedical optics express · 2019Article
- Candle Soot Carbon Nanoparticles in Photoacoustics: Advantages and Challenges for Laser Ultrasound Transmitters.IEEE nanotechnology magazine · 2019Article
- Microfluidic High-Migratory Cell Collector Suppressing Artifacts Caused by Microstructures.Micromachines · 2019Article
- Single-Cell Mobility Analysis of Metastatic Breast Cancer Cells.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2018Article
- Photoacoustic Energy Sensor for Nanosecond Optical Pulse Measurement.Sensors (Basel, Switzerland) · 2018Article
- Current Trends of Microfluidic Single-Cell Technologies.International journal of molecular sciences · 2018Review
- Review
Corrections and comments
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Authors and funding
13 authors at 3 institutions in 2 countries.
Funding
Abstract
Considerable evidence suggests that self-renewal and differentiation of cancer stem-like cells, a key cell population in tumorgenesis, can determine the outcome of disease. Though the development of microfluidics has enhanced the study of cellular lineage, it remains challenging to retrieve sister cells separately inside enclosed microfluidics for further analyses. In this work, we developed a photomechanical method to selectively detach and reliably retrieve target cells from enclosed microfluidic chambers. Cells cultured on carbon nanotube-polydimethylsiloxane composite surfaces can be detached using shear force induced through irradiation of a nanosecond-pulsed laser. This retrieval process has been verified to preserve cell viability, membrane proteins, and mRNA expression levels. Using the presented method, we have successfully performed 96-plex single-cell transcriptome analysis on sister cells in order to identify the genes altered during self-renewal and differentiation, demonstrating phenomenal resolution in the study of cellular lineage.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.