ArticleAfrican journal of traditional, complementary, and alternative medicines : AJTCAM2017
PURIFICATION AND FRACTIONAL ANALYSIS OF METHANOLIC EXTRACT OF
Article in African journal of traditional, complementary, and alternative medicines : AJTCAM, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
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Who cites it
2 citing papers in PubMed, 7 citations in OpenAlex.
- Antifungal efficacy ofBiochemistry and biophysics reports · 2021Article
- Microbial Production of Retinyl Palmitate and Its Application as a Cosmeceutical.Antioxidants (Basel, Switzerland) · 2020Article
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Authors and funding
3 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundMATERIALS AND
methodsThe methanolic extract of WT was initially purified through thin layer chromatography (TLC) and screened for the apoptotic and anti-leukemia activities. The positive band of TLC was subjected to silica gel column chromatography for further purification and the fractions obtained from it were screened again for anti-leukemia activity through thymidine uptake assay and apoptotic activity by DNA fragmentation, nuclear staining and flow cytometry assays. The fraction with positive result was subjected to HPLC for analysis of bioactive components.
resultsOut of many combinations of solvents, the methanol and dichloromethane combination in the ratio 6:4 has revealed two bands in TLC, among which the second band showed positive results for apoptotic and anti-leukemic activities. Further purification of second band through silica gel chromatography gave five fractions in which the 3
conclusionThe single peak revealed through HPLC indicates the presence of pure compound with apoptotic and anti-leukemia activities encouraging for further structural analysis.
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