Evidence map›Paper›PMID 28479376›Full record

ArticleGastroenterology2017

Extracellular Vesicles in Bile as Markers of Malignant Biliary Stenoses.

Valeria Severino, Jean-Marc Dumonceau, Myriam Delhaye, Solange Moll, Isabelle Annessi-Ramseyer, Xavier Robin, Jean-Louis Frossard, Annarita Farina

Abstract readEvaluation StudyMulticenter Study
PubMed Publisher
In one paragraph

Article in Gastroenterology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 59 papers.

0numbers the graph read from it
0cells of the map it votes in
59citing papers in PubMed
4.5field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

59 citing papers in PubMed, 97 citations in OpenAlex.

  1. Trial
  2. Review
  3. Review
  4. Review
  5. Cholangiocarcinoma: The era of liquid biopsy.World journal of gastroenterology · 2025
    Review
  6. Review
  7. Article
  8. Molecular Biomarkers in Cholangiocarcinoma: Focus on Bile.Current topics in medicinal chemistry · 2024
    Review
  9. Review
  10. Review
  11. Review
  12. Review
  13. Article
  14. Article
  15. Plasmon-Enhanced Single Extracellular Vesicle Analysis for Cholangiocarcinoma Diagnosis.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023
    Article
  16. Extracellular Vesicles as Biomarkers in Liver Disease.International journal of molecular sciences · 2022
    Review
  17. Review
  18. The focus clinical research in intrahepatic cholangiocarcinoma.European journal of medical research · 2022
    Review
  19. Review
  20. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

8 authors at 5 institutions in 4 countries.

Valeria SeverinoDepartment of Internal Medicine Specialties, University of Geneva, Geneva, Switzerland; Department of Human Protein Science, University of Geneva, Geneva, Switzerland.
Jean-Marc DumonceauGedyt Endoscopy Center, Buenos Aires, Argentina.
Myriam DelhayeDepartment of Gastroenterology, Hepatopancreatology and GI Oncology, Erasme University Hospital, Brussels, Belgium.
Solange MollDepartment of Pathology, University Hospitals of Geneva, Geneva, Switzerland.
Isabelle Annessi-RamseyerDepartment of Human Protein Science, University of Geneva, Geneva, Switzerland.
Xavier RobinBiotech Research and Innovation Center, University of Copenhagen, Copenhagen, Denmark.
Jean-Louis FrossardDepartment of Internal Medicine Specialties, University of Geneva, Geneva, Switzerland; Service of Gastroenterology and Hepatology, University Hospitals of Geneva, Switzerland.
Annarita FarinaDepartment of Internal Medicine Specialties, University of Geneva, Geneva, Switzerland; Department of Human Protein Science, University of Geneva, Geneva, Switzerland. Electronic address: Annarita.Farina@unige.ch.
University of Geneva · CHErasmus Hospital · BEGastroenterología Diagnóstica Terapeútica · ARUniversity Hospital of Geneva · CHUniversity of Copenhagen · DK

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

BACKGROUND &

aimsAlgorithms for diagnosis of malignant common bile duct (CBD) stenoses are complex and lack accuracy. Malignant tumors secrete large numbers of extracellular vesicles (EVs) into surrounding fluids; EVs might therefore serve as biomarkers for diagnosis. We investigated whether concentrations of EVs in bile could discriminate malignant from nonmalignant CBD stenoses.

methodsWe collected bile and blood samples from 50 patients undergoing therapeutic endoscopic retrograde cholangiopancreatography at university hospitals in Europe for CBD stenosis of malignant (pancreatic cancer, n = 20 or cholangiocarcinoma, n = 5) or nonmalignant (chronic pancreatitis [CP], n = 15) origin. Ten patients with CBD obstruction due to biliary stones were included as controls. EV concentrations in samples were determined by nanoparticle tracking analyses. The discovery cohort comprised the first 10 patients with a diagnosis of pancreatic cancer, based on tissue analysis, and 10 consecutive controls. Using samples from these subjects, we identified a threshold concentration of bile EVs that could best discriminate between patients with pancreatic cancer from controls. We verified the diagnostic performance of bile EV concentration by analyzing samples from the 30 consecutive patients with a diagnosis of malignant (pancreatic cancer or cholangiocarcinoma, n = 15) or nonmalignant (CP, n = 15) CBD stenosis. Samples were compared using the Mann-Whitney test and nonparametric Spearman correlation analysis. Receiver operating characteristic area under the curve was used to determine diagnostic accuracy.

resultsIn both cohorts, the median concentration of EVs was significantly higher in bile samples from patients with malignant CBD stenoses than controls or nonmalignant CBD stenoses (2.41 × 10

conclusionsConcentration of EVs in bile samples discriminates between patients with malignant vs nonmalignant CBD stenosis with 100% accuracy. Further studies are needed to confirm these findings. Clinical Trial registration no: ISRCTN66835592.

Indexed as

BileExtracellular VesiclesAdultAgedAged, 80 and overBile Duct NeoplasmsBiomarkers, TumorCholangiocarcinomaCholestasisEuropeFemaleGallstonesHumansMaleMiddle AgedPancreatic NeoplasmsBiomarkers, TumorBiliary StricturesExosomesMicroparticlesMicrovesicles

Identifiers

PMID28479376
OpenAlexW2611694969

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.