ArticleThe EMBO journal1988
Biosynthesis, secretion and extracellular localization of anchorin CII, a collagen-binding protein of the calpactin family.
Article in The EMBO journal, 1988. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 22 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
22 citing papers in PubMed, 123 citations in OpenAlex.
- ANXA5: related mechanisms of osteogenesis and additional biological functions.Frontiers in cell and developmental biology · 2025Review
- Novel blood coagulation molecules: Skeletal muscle myosin and cardiac myosin.Journal of thrombosis and haemostasis : JTH · 2021Review
- Annexin A5 regulates surface αvβ5 integrin for retinal clearance phagocytosis.Journal of cell science · 2019Article
- Annexin A5 is not essential for skeletal development.Molecular and cellular biology · 2003Article
- Functional analysis of the human annexin A5 gene promoter: a downstream DNA element and an upstream long terminal repeat regulate transcription.The Biochemical journal · 2001Article
- Fibroblasts contracting collagen matrices form transient plasma membrane passages through which the cells take up fluorescein isothiocyanate-dextran and Ca2+.Molecular biology of the cell · 1997Article
- Proteins in unexpected locations.Molecular biology of the cell · 1996Review
- Identification, localization, and functional implications of an abundant nematode annexin.The Journal of cell biology · 1996Article
- Annexins of plant cells.Plant physiology · 1995Review
- Glucocorticoid-induced annexin 1 secretion by monocytes and peritoneal leukocytes.British journal of pharmacology · 1995Article
- Cartilage collagens: strategies for the study of their organisation and expression in the extracellular matrix.Annals of the rheumatic diseases · 1994Review
- Annexin 5 as a potential regulator of annexin 1 phosphorylation by protein kinase C. In vitro inhibition compared with quantitative data on annexin distribution in human endothelial cells.The Biochemical journal · 1993Article
- Interferon-alpha and interleukin 2 synergistically enhance basic fibroblast growth factor synthesis and induce release, promoting endothelial cell growth.The Journal of clinical investigation · 1993Article
- Immunohistochemical localization of articular cartilage proteoglycan and link protein in situ using monoclonal antibodies and lectin-binding methods.Histochemistry · 1993Article
- Isolation, characterization and localization of annexin V from chicken liver.The Biochemical journal · 1993Article
- Glucocorticoid-and non-glucocorticoid induction of lipocortins (annexins) 1 and 2 in rat peritoneal leucocytes in vivo.British journal of pharmacology · 1993Article
- Expression of human recombinant lipocortin I in a wheat-germ cell-free system and Xenopus oocytes. Lipocortin is not secreted.The Biochemical journal · 1991Article
- Expression of anchorin CII mRNA by cultured chondrocytes.Cytotechnology · 1991Article
- Expression of annexin VI (p68, 67 kDa-calelectrin) in normal human tissues: evidence for developmental regulation in B- and T-lymphocytes.Histochemistry · 1991Article
- Evidence for export of a muscle lectin from cytosol to extracellular matrix and for a novel secretory mechanism.The Journal of cell biology · 1990Article
Corrections and comments
- Erratum issued
Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The amino acid sequence of anchorin CII, a collagen-binding protein isolated originally from chondrocyte membranes, was previously determined by sequencing of cDNA and proteolytic fragments of the protein. Computer analysis of the protein sequence revealed four internal repeats of approximately 70-80 residues, each containing a highly conserved consensus sequence of 17 residues. These repeats show considerable homology with sequences in human and bovine calpactin, lipocortin, endonexin and protein II, which are members of a family of Ca2+- and phospholipid-binding proteins, as well as major substrates of tyrosine kinases. While these proteins have been located at the inner side of the plasma membrane of fibroblasts and epithelial cells, here we present experimental evidence that anchorin CII is at least partially released from cells and binds to the outer cell surface. Biosynthesis studies in cell-free systems and in cell culture indicate that anchorin CII is not processed, which is consistent with the absence of signal sequences from the protein. Yet, pulse-chase experiments show that anchorin is released into the culture medium of fibroblasts after 30 min, and in chondrocyte cultures after 20 h. Anchorin CII was located to the outer cell surface of chondrocytes by lactoperoxidase-catalyzed cell surface iodination as well as by antibody labeling both at light- and electron-microscopical level. The pericellular localization of anchorin CII is consistent with the notion that this protein is involved in the interaction of chondrocytes and fibroblasts with extracellular collagen.
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