ArticleArchives of toxicology2017
Combination of multiple neural crest migration assays to identify environmental toxicants from a proof-of-concept chemical library.
Article in Archives of toxicology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Challenges and solutions in transitioning to animal-free standards: a comprehensive analysis of components in human cell-based developmental neurotoxicity assays.Frontiers in toxicology · 2026Review
- Testing Strategies for Metabolite-Mediated Neurotoxicity.International journal of molecular sciences · 2025Review
- 2024 International Academy of Toxicologic Pathology (IATP) Satellite Symposium: New Approach Methodologies (NAMs) for Neurotoxicity Assessment and Regulatory Perspectives.Toxicologic pathology · 2025Article
- Inhibition of Neural Crest Cell Migration by Strobilurin Fungicides and Other Mitochondrial Toxicants.Cells · 2024Article
- Article
- New approach methods to assess developmental and adult neurotoxicity for regulatory use: a PARC work package 5 project.Frontiers in toxicology · 2024Article
- Definition of the Neurotoxicity-Associated Metabolic Signature Triggered by Berberine and Other Respiratory Chain Inhibitors.Antioxidants (Basel, Switzerland) · 2023Article
- Neurodevelopmental toxicity assessment of flame retardants using a human DNT in vitro testing battery.Cell biology and toxicology · 2022Article
- Generation of Human Nociceptor-Enriched Sensory Neurons for the Study of Pain-Related Dysfunctions.Stem cells translational medicine · 2022Article
- Complex Elucidation of Cells-of-Origin in Pediatric Soft Tissue Sarcoma: From Concepts to Real Life, Hide-and-Seek through Epigenetic and Transcriptional Reprogramming.International journal of molecular sciences · 2022Review
- Profiling of Human Neural Crest Chemoattractant Activity as a Replacement of Fetal Bovine Serum for In Vitro Chemotaxis Assays.International journal of molecular sciences · 2021Article
- Endocrine disruptors also function as nervous disruptors and can be renamed endocrine and nervous disruptors (ENDs).Toxicology reports · 2021Review
- Optimization of Human Neural Progenitor Cells for an Imaging-Based High-Throughput Phenotypic Profiling Assay for Developmental Neurotoxicity Screening.Frontiers in toxicology · 2021Article
- A cross-platform approach to characterize and screen potential neurovascular unit toxicants.Reproductive toxicology (Elmsford, N.Y.) · 2020Article
- The multi-dimensional embryonic zebrafish platform predicts flame retardant bioactivity.Reproductive toxicology (Elmsford, N.Y.) · 2020Article
- The EU-ToxRisk method documentation, data processing and chemical testing pipeline for the regulatory use of new approach methods.Archives of toxicology · 2020Article
- Internationalization of read-across as a validated new approach method (NAM) for regulatory toxicology.ALTEX · 2020Article
- Sub-lethal effects of permethrin exposure on a passerine: implications for managing ectoparasites in wild bird nests.Conservation physiology · 2020Article
- Review of high-content screening applications in toxicology.Archives of toxicology · 2019Review
- Screening for Developmental Neurotoxicity at the National Toxicology Program: The Future Is Here.Toxicological sciences : an official journal of the Society of Toxicology · 2019Article
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Authors and funding
8 authors.
Funding
Abstract
Many in vitro tests have been developed to screen for potential neurotoxicity. However, only few cell function-based tests have been used for comparative screening, and thus experience is scarce on how to confirm and evaluate screening hits. We addressed these questions for the neural crest cell migration test (cMINC). After an initial screen, a hit follow-up strategy was devised. A library of 75 compounds plus internal controls (NTP80-list), assembled by the National Toxicology Program of the USA (NTP) was used. It contained some known classes of (developmental) neurotoxic compounds. The primary screen yielded 23 confirmed hits, which comprised ten flame retardants, seven pesticides and six drug-like compounds. Comparison of concentration-response curves for migration and viability showed that all hits were specific. The extent to which migration was inhibited was 25-90%, and two organochlorine pesticides (DDT, heptachlor) were most efficient. In the second part of this study, (1) the cMINC assay was repeated under conditions that prevent proliferation; (2) a transwell migration assay was used as a different type of migration assay; (3) cells were traced to assess cell speed. Some toxicants had largely varying effects between assays, but each hit was confirmed in at least one additional test. This comparative study allows an estimate on how confidently the primary hits from a cell function-based screen can be considered as toxicants disturbing a key neurodevelopmental process. Testing of the NTP80-list in more assays will be highly interesting to assemble a test battery and to build prediction models for developmental toxicity.
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