ArticleNucleic acids research2017
Sak and Sak4 recombinases are required for bacteriophage replication in Staphylococcus aureus.
Article in Nucleic acids research, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 17 citations in OpenAlex.
- Characterization of strictly lytic phages infectingMicrobiology spectrum · 2025Article
- The RecD2 helicase balances RecA activities.Nucleic acids research · 2022Article
- Cleavage of viral DNA by restriction endonucleases stimulates the type II CRISPR-Cas immune response.Molecular cell · 2022Article
- Phage satellites and their emerging applications in biotechnology.FEMS microbiology reviews · 2021Review
- Expanding Diversity ofFrontiers in microbiology · 2021Article
- The gp44 Ejection Protein ofViruses · 2020Article
- The structure of a polygamous repressor reveals how phage-inducible chromosomal islands spread in nature.Nature communications · 2019Article
- Article
- Sak4 of Phage HK620 Is a RecA Remote Homolog With Single-Strand Annealing Activity Stimulated by Its Cognate SSB Protein.Frontiers in microbiology · 2018Article
- Pirating conserved phage mechanisms promotes promiscuous staphylococcal pathogenicity island transfer.eLife · 2017Article
- Molecular Mechanisms That Contribute to Horizontal Transfer of Plasmids by the Bacteriophage SPP1.Frontiers in microbiology · 2017Article
Corrections and comments
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Authors and funding
9 authors at 5 institutions in 3 countries.
Funding
Abstract
DNA-single strand annealing proteins (SSAPs) are recombinases frequently encoded in the genome of many bacteriophages. As SSAPs can promote homologous recombination among DNA substrates with an important degree of divergence, these enzymes are involved both in DNA repair and in the generation of phage mosaicisms. Here, analysing Sak and Sak4 as representatives of two different families of SSAPs present in phages infecting the clinically relevant bacterium Staphylococcus aureus, we demonstrate for the first time that these enzymes are absolutely required for phage reproduction. Deletion of the genes encoding these enzymes significantly reduced phage replication and the generation of infectious particles. Complementation studies revealed that these enzymes are required both in the donor (after prophage induction) and in the recipient strain (for infection). Moreover, our results indicated that to perform their function SSAPs require the activity of their cognate single strand binding (Ssb) proteins. Mutational studies demonstrated that the Ssb proteins are also required for phage replication, both in the donor and recipient strain. In summary, our results expand the functions attributed to the Sak and Sak4 proteins, and demonstrate that both SSAPs and Ssb proteins are essential for the life cycle of temperate staphylococcal phages.
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Registered trials
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