ArticleFEMS yeast research2017
Sugar versus fat: elimination of glycogen storage improves lipid accumulation in Yarrowia lipolytica.
Article in FEMS yeast research, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
27 citing papers in PubMed, 1 synthesis or guideline pooled it, 63 citations in OpenAlex.
- Adipocyte Size, Overweight, and Insulin Resistance in Type 2 Diabetes Mellitus and the Impact of Weight Loss: A Systematic Review.Nutrients · 2026Pooled it
- Toward Complex Polyketide Biosynthesis inJournal of microbiology and biotechnology · 2026Review
- Engineering yeast for tailored fatty acid profiles.Applied microbiology and biotechnology · 2025Review
- Effect of Argan Oil on Lipid Production byJournal of microbiology and biotechnology · 2025Article
- Growth Response of Non-Conventional Yeasts on Sugar-Rich Media: Part 2: Citric Acid Production and Circular-Oriented Valorization of Glucose-Enriched Olive Mill Wastewaters Using NovelMicroorganisms · 2023Article
- AfLaeA, a Global Regulator of Mycelial Growth, Chlamydospore Production, Pathogenicity, Secondary Metabolism, and Energy Metabolism in the Nematode-Trapping FungusMicrobiology spectrum · 2023Article
- Genome Analysis of a Variant ofMicroorganisms · 2023Article
- Post-Consumer Poly(ethylene terephthalate) (PET) Depolymerization byMolecules (Basel, Switzerland) · 2022Article
- Lipid production from lignocellulosic biomass using an engineered Yarrowia lipolytica strain.Microbial cell factories · 2022Article
- Exploring Yeast Diversity to Produce Lipid-Based Biofuels from Agro-Forestry and Industrial Organic Residues.Journal of fungi (Basel, Switzerland) · 2022Review
- Production of human milk fat substitute by engineered strains ofMetabolic engineering communications · 2022Article
- High-Quality Genome Assembly ofFrontiers in plant science · 2022Article
- Production, Biosynthesis, and Commercial Applications of Fatty Acids From Oleaginous Fungi.Frontiers in nutrition · 2022Review
- The N-Acetylglucosamine Kinase fromInternational journal of molecular sciences · 2021Article
- Engineering of Saccharomyces cerevisiae for the accumulation of high amounts of triacylglycerol.Microbial cell factories · 2021Article
- The Role of Metabolic Engineering Technologies for the Production of Fatty Acids in Yeast.Biology · 2021Review
- Increasing lipid yield in Yarrowia lipolytica through phosphoketolase and phosphotransacetylase expression in a phosphofructokinase deletion strain.Biotechnology for biofuels · 2021Article
- Metabolic Engineering for Unusual Lipid Production inMicroorganisms · 2020Review
- Nitrogen as the major factor influencing gene expression inBiotechnology reports (Amsterdam, Netherlands) · 2020Article
- DES2 is a fatty acid Δ11 desaturase capable of synthesizing palmitvaccenic acid in the arbuscular mycorrhizal fungus Rhizophagus irregularis.FEBS letters · 2020Article
Corrections and comments
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Authors and funding
7 authors at 5 institutions in 2 countries.
Funding
Abstract
Triacylglycerol (TAG) and glycogen are the two major metabolites for carbon storage in most eukaryotic organisms. We investigated the glycogen metabolism of the oleaginous Yarrowia lipolytica and found that this yeast accumulates up to 16% glycogen in its biomass. Assuming that elimination of glycogen synthesis would result in an improvement of lipid accumulation, we characterized and deleted the single gene coding for glycogen synthase, YlGSY1. The mutant was grown under lipogenic conditions with glucose and glycerol as substrates and we obtained up to 60% improvement in TAG accumulation compared to the wild-type strain. Additionally, YlGSY1 was deleted in a background that was already engineered for high lipid accumulation. In this obese background, TAG accumulation was also further increased. The highest lipid content of 52% was found after 3 days of cultivation in nitrogen-limited glycerol medium. Furthermore, we constructed mutants of Y. lipolytica and Saccharomyces cerevisiae that are deleted for both glycogen and TAG synthesis, demonstrating that the ability to store carbon is not essential. Overall, this work showed that glycogen synthesis is a competing pathway for TAG accumulation in oleaginous yeasts and that deletion of the glycogen synthase has beneficial effects on neutral lipid storage.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.