ArticlePloS one2017
Low concentration DNA extraction and recovery using a silica solid phase.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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Who cites it
47 citing papers in PubMed.
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- Intimate Interaction Between Nucleic Acid and Conjugated Polymers in Organic Electrochemical Transistors Enables Ultrasensitive Biomarker Detection.Advanced materials (Deerfield Beach, Fla.) · 2026Article
- Quantitative analysis of sequential nucleic acid elution from silica paramagnetic beads.The Analyst · 2026Article
- Development of a one-tube RPA/Cas12a platform for dual-mode detection of Vibrio alginolyticus.World journal of microbiology & biotechnology · 2026Article
- Proof of concept for aqueous two-phase system-based extraction of cell-free DNA from plasma for liquid biopsy applications.Scientific reports · 2026Article
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- Zwitterionic Poly(sulfobetaine methacrylate) Brushes Functionalized Threads for DNA Extraction from Complex Cell Lysates.Sensors (Basel, Switzerland) · 2025Article
- A rapid and high-yield method for nucleic acid extraction.Scientific reports · 2025Article
- An affordable and simple method for DNA extraction from agarose suitable for downstream applications.Scientific reports · 2025Article
- Studying Cationic Liposomes for Quick, Simple, and Effective Nucleic Acid Preconcentration and Isolation.Analytical chemistry · 2025Article
- A rapid purification method for trace amounts of cell-free DNA in urine.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2025Article
- Evaluation of four DNA extraction kits for implementation of nanopore sequencing in routine surveillance of antimicrobial resistance in low-resource settings.Frontiers in microbiology · 2025Article
- DNA extraction from long-term preserved formalin-fixed paraffin-embedded samples of thymic epithelial tumours - a performance comparison of three commercial kits.Contemporary oncology (Poznan, Poland) · 2025Article
- Dried Blood Matrix as a New Material for the Detection of DNA Viruses.Advanced healthcare materials · 2024Article
- Affinity molecular assay for detecting Candida albicans using chitin affinity and RPA-CRISPR/Cas12a.Nature communications · 2024Article
- Detection ofMicroorganisms · 2024Article
- Optimization of a Protocol for Isolating Cell-free DNA From Cerebrospinal Fluid.Annals of laboratory medicine · 2024Article
- Non-Microbiological Mycobacterial Detection Techniques for Quality Control of Biological Products: A Comprehensive Review.Microorganisms · 2024Review
- An All-in-One Platform for On-Site Multiplex Foodborne Pathogen Detection Based on Channel-Digital Hybrid Microfluidics.Biosensors · 2024Article
- A high-performance polymer composite column for coronavirus nucleic acid purification.Scientific reports · 2024Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
DNA extraction from clinical samples is commonly achieved with a silica solid phase extraction column in the presence of a chaotrope. Versions of these protocols have been adapted for point of care (POC) diagnostic devices in miniaturized platforms, but commercial kits require a high amount of input DNA. Thus, when the input clinical sample contains less than 1 μg of total DNA, the target-specific DNA recovery from most of these protocols is low without supplementing the sample with exogenous carrier DNA. In fact, many clinical samples used in the development of POC diagnostics often exhibit target DNA concentrations as low as 3 ng/mL. With the broader goal of improving the yield and efficiency of nucleic acid-based POC devices for dilute samples, we investigated both DNA adsorption and recovery from silica particles by using 1 pg- 1 μg of DNA with a set of adsorption and elution buffers ranging in pH and chaotropic presence. In terms of adsorption, we found that low pH and the presence of chaotropic guanidinium thiocyanate (GuSCN) enhanced DNA-silica adsorption. When eluting with a standard low-salt, high-pH buffer, > 70% of DNA was unrecoverable, except when DNA was initially adsorbed with 5 M GuSCN at pH 5.2. Unrecovered DNA was either not initially adsorbed or irreversibly bound on the silica surface. Recovery was improved when eluting with 95°C formamide and 1 M NaOH, which suggested that DNA-silica-chaotrope interactions are dominated by hydrophobic interactions and hydrogen bonding. While heated formamide and NaOH are non-ideal elution buffers for practical POC devices, the salient results are important for engineering a set of optimized reagents that could maximize nucleic acid recovery from a microfluidic DNA-silica-chaotrope system.
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