ArticlePloS one2017
An improved method with high sensitivity and low background in detecting low β-galactosidase expression in mouse embryos.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed, 7 citations in OpenAlex.
- Evidence for Fgf and Wnt regulation ofFrontiers in cell and developmental biology · 2025Article
- Identifying vitamin A signaling by visualizing gene and protein activity, and by quantification of vitamin A metabolites.Methods in enzymology · 2020Article
- An Improved Staining Method for Low SignalBio-protocol · 2019Article
- Tracing Gene Expression Through Detection of β-galactosidase Activity in Whole Mouse Embryos.Journal of visualized experiments : JoVE · 2018Article
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
LacZ is widely used as a reporter in studies of gene expression patterns. β-galactosidase, the product of LacZ gene, is usually detected by X-gal/FeCN staining. In X-gal/FeCN staining, β-galactosidase catalyzes X-gal to produce blue precipitates, which indicate the expression patterns of the gene of interest. A newer LacZ detection method using S-gal/TNBT is more sensitive but plagued by high background. Here, we describe an improved procedure that combines advantageous steps from the two methods. By comparing with X-gal/FeCN and S-gal/TNBT methods in detecting the expression patterns of miR-322/503 and miR-451 at a series of developmental stages, the improved method showed higher sensitivity and lower background. Thus, the improved method could be an alternative way of β-galactosidase staining in low gene expression situations.
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