ArticleAnalytical chemistry2017
Label-Free Quantification of Intracellular Mitochondrial Dynamics Using Dielectrophoresis.
Article in Analytical chemistry, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed.
- Dielectric double shell characterization of yeast cells exposed to simulated microgravity.NPJ microgravity · 2026Article
- The Multi-Conductivity Clausius-Mossotti Factor as an Electrophysiology Rosetta Stone: Dielectrophoresis, Membrane Potential and Zeta Potential.Micromachines · 2025Review
- On-Chip Integration of Impedance Cytometry for Inline Optimization of Dielectrophoretic Separations on Multiple Cellular Biophysical Metrics.ACS sensors · 2025Article
- Electrical Phenotyping of Aged Human Mesenchymal Stem Cells Using Dielectrophoresis.Micromachines · 2025Article
- Quantifying the Electrophysiological Properties of Cells Using Dielectrophoresis.Methods in molecular biology (Clifton, N.J.) · 2025Article
- High-Frequency Dielectrophoresis Reveals That Distinct Bio-Electric Signatures of Colorectal Cancer Cells Depend on Ploidy and Nuclear Volume.Micromachines · 2023Article
- [Advances in microchip electrophoresis for the separation and analysis of biological samples].Se pu = Chinese journal of chromatography · 2023Review
- Supervised learning on impedance cytometry data for label-free biophysical distinction of pancreatic cancer cells versus their associated fibroblasts under gemcitabine treatment.Biosensors & bioelectronics · 2023Article
- Virtual labeling of mitochondria in living cells using correlative imaging and physics-guided deep learning.Biomedical optics express · 2022Article
- On-chip microfluidic buffer swap of biological samples in-line with downstream dielectrophoresis.Electrophoresis · 2022Article
- Modified Red Blood Cells as Multimodal Standards for Benchmarking Single-Cell Cytometry and Separation Based on Electrical Physiology.Analytical chemistry · 2022Article
- Deep-Learning Based Estimation of Dielectrophoretic Force.Micromachines · 2021Article
- Particle trapping in electrically driven insulator-based microfluidics: Dielectrophoresis and induced-charge electrokinetics.Electrophoresis · 2021Review
- Apoptotic Bodies in the Pancreatic Tumor Cell Culture Media Enable Label-Free Drug Sensitivity Assessment by Impedance Cytometry.Advanced biology · 2021Article
- Methods of Generating Dielectrophoretic Force for Microfluidic Manipulation of Bioparticles.ACS biomaterials science & engineering · 2021Review
- Self-aligned sequential lateral field non-uniformities over channel depth for high throughput dielectrophoretic cell deflection.Lab on a chip · 2021Article
- Microscale nonlinear electrokinetics for the analysis of cellular materials in clinical applications: a review.Mikrochimica acta · 2021Review
- Single-cell microfluidic impedance cytometry: from raw signals to cell phenotypes using data analytics.Lab on a chip · 2021Article
- Mito Hacker: a set of tools to enable high-throughput analysis of mitochondrial network morphology.Scientific reports · 2020Article
- High-throughput dynamical analysis of dielectrophoretic frequency dispersion of single cells based on deflected flow streamlines.Analytical and bioanalytical chemistry · 2020Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Mitochondrial dynamics play an important role within several pathological conditions, including cancer and neurological diseases. For the purpose of identifying therapies that target aberrant regulation of the mitochondrial dynamics machinery and characterizing the regulating signaling pathways, there is a need for label-free means to detect the dynamic alterations in mitochondrial morphology. We present the use of dielectrophoresis for label-free quantification of intracellular mitochondrial modifications that alter cytoplasmic conductivity, and these changes are benchmarked against label-based image analysis of the mitochondrial network. This is validated by quantifying the mitochondrial alterations that are carried out by entirely independent means on two different cell lines: human embryonic kidney cells and mouse embryonic fibroblasts. In both cell lines, the inhibition of mitochondrial fission that leads to a mitochondrial structure of higher connectivity is shown to substantially enhance conductivity of the cell interior, as apparent from the significantly higher positive dielectrophoresis levels in the 0.5-15 MHz range. Using single-cell velocity tracking, we show ∼10-fold higher positive dielectrophoresis levels at 0.5 MHz for cells with a highly connected versus those with a highly fragmented mitochondrial structure, suggesting the feasibility for frequency-selective dielectrophoretic isolation of cells to aid the discovery process for development of therapeutics targeting the mitochondrial machinery.
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Registered trials
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