Evidence map›Paper›PMID 28473986›Full record

ArticleBioMed research international2017

A Robust PCR Protocol for HIV Drug Resistance Testing on Low-Level Viremia Samples.

Shivani Gupta, Tracy Taylor, Aileen Patterson, Binhua Liang, Jared Bullard, Paul Sandstrom, Gary Van Domselaar, Hezhao Ji

Abstract read
In one paragraph

Article in BioMed research international, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Shivani GuptaDepartment of Medical Microbiology, University of Manitoba, Winnipeg, MB, Canada.ORCID 0000-0003-4865-8920
Tracy TaylorNational HIV & Retrovirology Laboratories, JC Wilt Infectious Diseases Research Center, Public Health Agency of Canada, Winnipeg, MB, Canada.
Aileen PattersonNational HIV & Retrovirology Laboratories, JC Wilt Infectious Diseases Research Center, Public Health Agency of Canada, Winnipeg, MB, Canada.ORCID 0000-0002-3761-5573
Binhua LiangThe Bioinformatics Core, National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, MB, Canada.
Jared BullardDepartment of Medical Microbiology, University of Manitoba, Winnipeg, MB, Canada.
Paul SandstromDepartment of Medical Microbiology, University of Manitoba, Winnipeg, MB, Canada.
Gary Van DomselaarDepartment of Medical Microbiology, University of Manitoba, Winnipeg, MB, Canada.
Hezhao JiDepartment of Medical Microbiology, University of Manitoba, Winnipeg, MB, Canada.ORCID 0000-0002-5451-6294

Funding

LAB SUP. FOR AIDS VACCINE &OTHER PREVENT. CLINICAL TRIAN01AI085341 · NIAID · SERACARE BIOSERVICES · PI COSENTINO, MARK · 1998 to 2000
$750k
NIAID NIH HHS N01 AI085341
6 · The paper itself

Abstract

The prevalence of drug resistance (DR) mutations in people with HIV-1 infection, particularly those with low-level viremia (LLV), supports the need to improve the sensitivity of amplification methods for HIV DR genotyping in order to optimize antiretroviral regimen and facilitate HIV-1 DR surveillance and relevant research. Here we report on a fully validated PCR-based protocol that achieves consistent amplification of the protease (PR) and reverse transcriptase (RT) regions of HIV-1

Indexed as

Drug Resistance, ViralGenotypeHIV-1HIV InfectionsHumansMutationpol Gene Products, Human Immunodeficiency VirusPolymerase Chain ReactionRNA, ViralViral LoadViremiapol Gene Products, Human Immunodeficiency VirusRNA, Viral

Identifiers

PMID28473986
PMCPMC5394351

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.