Evidence map›Paper›PMID 28473465›Full record

ArticleThe Journal of biological chemistry2017

Expression, purification, and spectral tuning of RhoGC, a retinylidene/guanylyl cyclase fusion protein and optogenetics tool from the aquatic fungus

Melissa M Trieu, Erin L Devine, Lindsey B Lamarche, Aaron E Ammerman, Jordan A Greco, Robert R Birge, Douglas L Theobald, Daniel D Oprian

Abstract read
In one paragraph

Article in The Journal of biological chemistry, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.

0numbers the graph read from it
0cells of the map it votes in
15citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

15 citing papers in PubMed.

  1. Diversity of rhodopsin cyclases in zoospore-forming fungi.Proceedings of the National Academy of Sciences of the United States of America · 2023
    Article
  2. Article
  3. Microbial Rhodopsins.Methods in molecular biology (Clifton, N.J.) · 2022
    Article
  4. Article
  5. Article
  6. Molecular Properties and Optogenetic Applications of Enzymerhodopsins.Advances in experimental medicine and biology · 2021
    Review
  7. Article
  8. Article
  9. Article
  10. Article
  11. Review
  12. Article
  13. Article
  14. Article
  15. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Melissa M TrieuFrom the Department of Biochemistry, Brandeis University, Waltham, Massachusetts 02454 and.
Erin L DevineFrom the Department of Biochemistry, Brandeis University, Waltham, Massachusetts 02454 and.
Lindsey B LamarcheFrom the Department of Biochemistry, Brandeis University, Waltham, Massachusetts 02454 and.
Aaron E AmmermanFrom the Department of Biochemistry, Brandeis University, Waltham, Massachusetts 02454 and.
Jordan A Grecothe Departments of Chemistry and Molecular and Cell Biology, University of Connecticut, Storrs, Connecticut 06269.
Robert R Birgethe Departments of Chemistry and Molecular and Cell Biology, University of Connecticut, Storrs, Connecticut 06269.
Douglas L TheobaldFrom the Department of Biochemistry, Brandeis University, Waltham, Massachusetts 02454 and dtheobald@brandeis.edu.ORCID 0000-0002-2695-8343
Daniel D OprianFrom the Department of Biochemistry, Brandeis University, Waltham, Massachusetts 02454 and oprian@brandeis.edu.ORCID 0000-0002-6520-5459

Funding

STRUCTURE/FUNCTION STUDIES OF RHODOPSINR01EY007965 · NEI · BRANDEIS UNIVERSITY · PI OPRIAN, DANIEL D · 1989 to 2017
$6.2M
Macromolecular Structure MechanismT32GM007596 · NIGMS · BRANDEIS UNIVERSITY · PI OPRIAN, DANIEL D · 1985 to 2019
$4.5M
Evolution of enzyme structure and function viewed at atomic resolutionR01GM096053 · NIGMS · BRANDEIS UNIVERSITY · PI Douglas Lowell Theobald · 2011 to 2026
$3.6M
PHOTOPHYSICS OF VISUAL CHROMOPHORES AND RHODOPSINR01GM034548 · NIGMS · UNIVERSITY OF CONNECTICUT STORRS · PI BIRGE, ROBERT RICHARDS · 1985 to 2012
$1.9M
Bayesian analysis of structural shape and conformationR01GM094468 · NIGMS · BRANDEIS UNIVERSITY · PI THEOBALD, DOUGLAS LOWELL · 2010 to 2014
$1.4M
NEI NIH HHS R01 EY007965NIGMS NIH HHS R01 GM034548NIGMS NIH HHS R01 GM094468NIGMS NIH HHS R01 GM096053NIGMS NIH HHS T32 GM007596
6 · The paper itself

Abstract

RhoGC is a rhodopsin (Rho)-guanylyl cyclase (GC) gene fusion molecule that is central to zoospore phototaxis in the aquatic fungus

Indexed as

BlastocladiomycotaFungal ProteinsGene ExpressionRecombinant Fusion ProteinsAmino Acid SubstitutionMutation, MissenseOptogeneticsProtein DomainsFungal ProteinsRecombinant Fusion Proteinsadenylate cyclase (adenylyl cyclase)cyclic nucleotideoptogeneticsphotoreceptorrhodopsinspectral tuning

Identifiers

PMID28473465
PMCPMC5481551

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.