ArticleNucleic acids research2017
Piwi interacts with chromatin at nuclear pores and promiscuously binds nuclear transcripts in Drosophila ovarian somatic cells.
Article in Nucleic acids research, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 48 citations in OpenAlex.
- Molecular insights into the Drosophila piRNA pathway via systematic ReLo protein interaction screening and structure prediction.Nucleic acids research · 2025Article
- Article
- Insulator BEAF32 regulates expression of tissue-specific genes and piRNA source loci in Drosophila ovaries.Epigenetics & chromatin · 2025Article
- Nucleoporin Elys attaches peripheral chromatin to the nuclear pores in interphase nuclei.Communications biology · 2024Article
- Reproducible evaluation of transposable element detectors with McClintock 2 guides accurate inference of Ty insertion patterns in yeast.Mobile DNA · 2023Article
- Reproducible evaluation of transposable element detectors with McClintock 2 guides accurate inference of Ty insertion patterns in yeast.bioRxiv : the preprint server for biology · 2023Article
- Comparison of genome architecture at two stages of male germline cell differentiation in Drosophila.Nucleic acids research · 2022Article
- Impaired function of rDNA transcription initiation machinery leads to derepression of ribosomal genes with insertions of R2 retrotransposon.Nucleic acids research · 2022Article
- Channel nuclear pore protein 54 directs sexual differentiation and neuronal wiring of female reproductive behaviors in Drosophila.BMC biology · 2021Article
- piRNA- and siRNA-mediated transcriptional repression in Drosophila, mice, and yeast: new insights and biodiversity.EMBO reports · 2021Review
- Genome-wide mapping of Piwi association with specific loci in Drosophila ovaries.G3 (Bethesda, Md.) · 2021Article
- Tissue-specific DamID protocol using nanopore sequencing.Journal of biological methods · 2021Article
- Article
- The Role of Nucleoporin Elys in Nuclear Pore Complex Assembly and Regulation of Genome Architecture.International journal of molecular sciences · 2020Review
- Various modes of HP1a interactions with the euchromatic chromosome arms in Drosophila ovarian somatic cells.Chromosoma · 2020Article
- PIWI-piRNA pathway-mediated transposable element repression inRNA (New York, N.Y.) · 2020Article
- Article
- Special vulnerability of somatic niche cells to transposable element activation in Drosophila larval ovaries.Scientific reports · 2020Article
- Nuclear Ccr4-Not mediates the degradation of telomeric and transposon transcripts at chromatin in the Drosophila germline.Nucleic acids research · 2020Article
- The piRNA pathway in Drosophila ovarian germ and somatic cells.Proceedings of the Japan Academy. Series B, Physical and biological sciences · 2020Review
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Authors and funding
13 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Piwi in a complex with Piwi-interacting RNAs (piRNAs) triggers transcriptional silencing of transposable elements (TEs) in Drosophila ovaries, thus ensuring genome stability. To do this, Piwi must scan the nascent transcripts of genes and TEs for complementarity to piRNAs. The mechanism of this scanning is currently unknown. Here we report the DamID-seq mapping of multiple Piwi-interacting chromosomal domains in somatic cells of Drosophila ovaries. These domains significantly overlap with genomic regions tethered to Nuclear Pore Complexes (NPCs). Accordingly, Piwi was coimmunoprecipitated with the component of NPCs Elys and with the Xmas-2 subunit of RNA transcription and export complex, known to interact with NPCs. However, only a small Piwi fraction has transient access to DNA at nuclear pores. Importantly, although 36% of the protein-coding genes overlap with Piwi-interacting domains and RNA-immunoprecipitation results demonstrate promiscuous Piwi binding to numerous genic and TE nuclear transcripts, according to available data Piwi does not silence these genes, likely due to the absence of perfect base-pairing between piRNAs and their transcripts.
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