ArticleNucleic acids research2017
Precise integration of inducible transcriptional elements (PrIITE) enables absolute control of gene expression.
Article in Nucleic acids research, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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20 citing papers in PubMed, 26 citations in OpenAlex.
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- Mammalian cell-based production of glycans, glycopeptides and glycomodules.Nature communications · 2024Article
- Site-Specific Integration by Circular Donor Improves CRISPR/Cas9-Mediated Homologous Recombination in Human Cell Lines.International journal of molecular sciences · 2024Article
- Machine learning-based screening and validation of liver metastasis-specific genes in colorectal cancer.Scientific reports · 2024Article
- The SHDRA syndrome-associated geneProceedings of the National Academy of Sciences of the United States of America · 2023Article
- CDX2 regulates interleukin-33 gene expression in intestinal epithelial cells (LS174T).FEBS open bio · 2021Article
- Probing the binding specificities of human Siglecs by cell-based glycan arrays.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- A panel of intestinal differentiation markers (CDX2, GPA33, and LI-cadherin) identifies gastric cancer patients with favourable prognosis.Gastric cancer : official journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association · 2020Article
- CDX2 expression and perioperative patient serum affects the adhesion properties of cultured colon cancer cells.BMC cancer · 2020Article
- A validated collection of mouse monoclonal antibodies to human glycosyltransferases functioning in mucin-type O-glycosylation.Glycobiology · 2019Article
- An Atlas of Human Glycosylation Pathways Enables Display of the Human Glycome by Gene Engineered Cells.Molecular cell · 2019Article
- HNF4α and CDX2 Regulate IntestinalInternational journal of molecular sciences · 2019Article
- A strategy for generating cancer-specific monoclonal antibodies to aberrant O-glycoproteins: identification of a novel dysadherin-Tn antibody.Glycobiology · 2019Article
- Probing the contribution of individual polypeptide GalNAc-transferase isoforms to theThe Journal of biological chemistry · 2018Article
- Intestinal regulation of suppression of tumorigenicity 14 (ST14) and serine peptidase inhibitor, Kunitz type -1 (SPINT1) by transcription factor CDX2.Scientific reports · 2018Article
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- Design and implementation of a synthetic pre-miR switch for controlling miRNA biogenesis in mammals.Nucleic acids research · 2017Article
- Genetic glycoengineering in mammalian cells.The Journal of biological chemistryReview
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Authors and funding
11 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Tetracycline-based inducible systems provide powerful methods for functional studies where gene expression can be controlled. However, the lack of tight control of the inducible system, leading to leakiness and adverse effects caused by undesirable tetracycline dosage requirements, has proven to be a limitation. Here, we report that the combined use of genome editing tools and last generation Tet-On systems can resolve these issues. Our principle is based on precise integration of inducible transcriptional elements (coined PrIITE) targeted to: (i) exons of an endogenous gene of interest (GOI) and (ii) a safe harbor locus. Using PrIITE cells harboring a GFP reporter or CDX2 transcription factor, we demonstrate discrete inducibility of gene expression with complete abrogation of leakiness. CDX2 PrIITE cells generated by this approach uncovered novel CDX2 downstream effector genes. Our results provide a strategy for characterization of dose-dependent effector functions of essential genes that require absence of endogenous gene expression.
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