ArticleNucleic acids research2017
The 2-μm plasmid encoded protein Raf1 regulates both stability and copy number of the plasmid by blocking the formation of the Rep1-Rep2 repressor complex.
Article in Nucleic acids research, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 17 citations in OpenAlex.
- pSB3, a member of the 2-micron family of plasmids, replicates and is efficiently partitioned in multiple budding yeast species.G3 (Bethesda, Md.) · 2026Article
- A hybrid system enables plasmid copy number control in yeast.Nature communications · 2026Article
- Late gene regulation by the alternative sigma factors ofmSystems · 2025Article
- Evidence of 14-3-3 proteins contributing to kinetochore integrity and chromosome congression during mitosis.Journal of cell science · 2024Article
- The yeast 2-micron plasmid Rep2 protein has Rep1-independent partitioning function.Nucleic acids research · 2022Article
- The selfish yeast plasmid utilizes the condensin complex and condensed chromatin for faithful partitioning.PLoS genetics · 2021Article
- Harnessing the Endogenous 2μ Plasmid ofFrontiers in microbiology · 2021Article
- Endogenous 2μ Plasmid Editing for Pathway Engineering inFrontiers in microbiology · 2021Article
- A Flp-SUMO hybrid recombinase reveals multi-layered copy number control of a selfish DNA element through post-translational modification.PLoS genetics · 2019Article
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The 2-μm plasmid of the budding yeast Saccharomyces cerevisiae achieves a high chromosome-like stability with the help of four plasmid-encoded (Rep1, Rep2, Raf1 and Flp) and several host-encoded proteins. Rep1 and Rep2 and the DNA locus STB form the partitioning system ensuring equal segregation of the plasmid. The Flp recombinase and its target sites FRTs form the amplification system which is responsible for the steady state plasmid copy number. In this work we show that the absence of Raf1 can affect both the plasmid stability and the steady sate copy number. We also show that the Rep proteins do bind to the promoter regions of the 2-μm encoded genes, as predicted by earlier models and Raf1 indeed blocks the formation of the Rep1-Rep2 repressor complex not by blocking the transcription of the REP1 and REP2 genes but by physically associating with the Rep proteins and negating their interactions. This explains the role of Raf1 in both the partitioning and the amplification systems as the Rep1-Rep2 complex is believed to modulate both these systems. Based on this study, we have provided, from a systems biology perspective, a model for the mechanism of the 2-μm plasmid maintenance.
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