ArticleMolecular biology and evolution2017
Deciphering the Genic Basis of Yeast Fitness Variation by Simultaneous Forward and Reverse Genetics.
Article in Molecular biology and evolution, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed.
- Natural selection shapes the evolution of the metabolome in Saccharomyces cerevisiae.Molecular biology and evolution · 2026Article
- Contrasting selective signatures of gene expression plasticity in response to antifungal clotrimazole and common environmental stresses in yeast.Nature communications · 2026Article
- The structure of an ancient genotype-phenotype map shaped the functional evolution of a protein family.Nature ecology & evolution · 2025Article
- The structure of an ancient genotype-phenotype map shaped the functional evolution of a protein family.bioRxiv : the preprint server for biology · 2025Article
- Horizontal Transfer and Recombination Fuel Ty4 Retrotransposon Evolution in Saccharomyces.Genome biology and evolution · 2025Article
- Overview of the Saccharomyces cerevisiae population structure through the lens of 3,034 genomes.G3 (Bethesda, Md.) · 2024Article
- Further Evidence for Strong Nonneutrality of Yeast Synonymous Mutations.Molecular biology and evolution · 2024Article
- Patterns and evolutionary consequences of pleiotropy.Annual review of ecology, evolution, and systematics · 2023Article
- Genome-wide probing of eukaryotic nascent RNA structure elucidates cotranscriptional folding and its antimutagenic effect.Nature communications · 2023Article
- Article
- Contributions of mutation and selection to regulatory variation: lessons from thePhilosophical transactions of the Royal Society of London. Series B, Biological sciences · 2023Review
- A thousand-genome panel retraces the global spread and adaptation of a major fungal crop pathogen.Nature communications · 2023Article
- Network Topology Can Explain Differences in Pleiotropy Between Cis- and Trans-regulatory Mutations.Molecular biology and evolution · 2022Article
- High-throughput approaches to functional characterization of genetic variation in yeast.Current opinion in genetics & development · 2022Review
- Pleiotropic effects of trans-regulatory mutations on fitness and gene expression.Science (New York, N.Y.) · 2022Article
- Epistatic drift causes gradual decay of predictability in protein evolution.Science (New York, N.Y.) · 2022Article
- Transposon insertional mutagenesis of diverse yeast strains suggests coordinated gene essentiality polymorphisms.Nature communications · 2022Article
- Fluorescence lifetime imaging of NAD(P)H upon oxidative stress inFrontiers in bioengineering and biotechnology · 2022Article
- The rate and molecular spectrum of mutation are selectively maintained in yeast.Nature communications · 2021Article
- Asexual Experimental Evolution of Yeast Does Not Curtail Transposable Elements.Molecular biology and evolution · 2021Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
The budding yeast Saccharomyces cerevisiae is the best studied eukaryote in molecular and cell biology, but its utility for understanding the genetic basis of phenotypic variation in natural populations is limited by inefficient association mapping due to strong and complex population structure. To overcome this challenge, we generated genome sequences for 85 strains and performed a comprehensive population genomic survey of a total of 190 diverse strains. We identified considerable variation in population structure among chromosomes and identified 181 genes that are absent from the reference genome. Many of these nonreference genes are expressed and we functionally confirmed that two of these genes confer increased resistance to antifungals. Next, we simultaneously measured the growth rates of over 4,500 laboratory strains, each of which lacks a nonessential gene, and 81 natural strains across multiple environments using unique DNA barcode present in each strain. By combining the genome-wide reverse genetic information gained from the gene deletion strains with a genome-wide association analysis from the natural strains, we identified genomic regions associated with fitness variation in natural populations. To experimentally validate a subset of these associations, we used reciprocal hemizygosity tests, finding that while the combined forward and reverse genetic approaches can identify a single causal gene, the phenotypic consequences of natural genetic variation often follow a complicated pattern. The resources and approach provided outline an efficient and reliable route to association mapping in yeast and significantly enhance its value as a model for understanding the genetic mechanisms underlying phenotypic variation and evolution in natural populations.
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