ArticleBioengineered2017
A synthetic hybrid promoter for D-xylonate production at low pH in the tolerant yeast Candida glycerinogenes.
Article in Bioengineered, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed, 16 citations in OpenAlex.
- Wine Barrel Biofilm as a Source of Yeasts with Non-Conventional Properties.Microorganisms · 2024Article
- Synthesis of pinene in the industrial strain Candida glycerinogenes by modification of its mevalonate pathway.Journal of microbiology (Seoul, Korea) · 2022Article
- Recent progress in the microbial production of xylonic acid.World journal of microbiology & biotechnology · 2022Review
- Sequential Production of ᴅ-xylonate and Ethanol from Non-Detoxified Corncob at Low-pH byJournal of fungi (Basel, Switzerland) · 2021Article
- Transcription factor Hap5 induces gsh2 expression to enhance 2-phenylethanol tolerance and production in an industrial yeast Candida glycerinogenes.Applied microbiology and biotechnology · 2020Article
- Systems and Synthetic Biology Approaches to Engineer Fungi for Fine Chemical Production.Frontiers in bioengineering and biotechnology · 2018Review
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Authors and funding
4 authors at 1 institution in 1 country.
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Abstract
The tolerant yeast Candida glycerinogenes, with high D-xylonate and low-pH tolerances, was used as the host for D-xylonate production at low pH in this study. A low-pH inducible promoter, pGUKd, was engineered using the core promoter of the glyceraldehyde-3-phosphate dehydrogenase gene (pGAP) combined with the upstream activating sequence of the promoter of the guanylate kinase gene (pGUK1) that had substituted pH-responsive TF binding sites. The recombinant cells that expressed GFP from the hybrid promoter pGUKd displayed dramatically increased fluorescence intensity at pH 2.5, thus verifying that pGUKd is a low-pH inducible promoter. The promoter pGUKd was then used to express the D-xylose dehydrogenase gene xylB, resulting in increased expression levels of xylB at low pH. The recombinant protein exhibited higher specific activities under lower pH conditions and produced 38 g/l D-xylonate at pH 2.5. This rate is much higher than that produced by fermentation at pH 5.5. These results suggest that the novel hybrid promoter pGUKd functions to direct the production of D-xylonate at low pH, and we provide a candidate genetic tool for the stress tolerant yeast C. glycerinogenes.
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