ArticleAmerican journal of translational research2017
Methylation of kruppel-like factor 2 (KLF2) associates with its expression and non-small cell lung cancer progression.
Article in American journal of translational research, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 37 citations in OpenAlex.
- Guardians of silence: transcriptional networks in T cell quiescence.Experimental & molecular medicine · 2025Review
- STAU1 exhibits oncogenic characteristics and modulates alternative splicing and gene expression in lung adenocarcinoma cells.Scientific reports · 2025Article
- Hypoxia LUAD H1975 cell-derived exosomal miR-671-3p promotes angiogenesis via regulating KLF2-VEGFR2 axis.Scientific reports · 2025Article
- Abnormal expression of Krüppel-like transcription factors and their potential values in lung cancer.Heliyon · 2024Review
- KLF2 controls proliferation and apoptosis of human spermatogonial stem cells via targeting GJA1.iScience · 2024Article
- Mechanobiology of portal hypertension.JHEP reports : innovation in hepatology · 2023Review
- PRC2 mediated KLF2 down regulation: a therapeutic and diagnostic axis during tumor progression.Cancer cell international · 2023Review
- Circ_0043256 upregulates KLF2 expression by absorbing miR-1206 to suppress the tumorigenesis of lung cancer.Thoracic cancer · 2023Article
- Lung Cancer Gene Regulatory Network of Transcription Factors Related to the Hallmarks of Cancer.Current issues in molecular biology · 2023Article
- Bioinformatics analysis of KLF2 as a potential prognostic factor in ccRCC and association with epithelial-mesenchymal transition.Experimental and therapeutic medicine · 2022Article
- Article
- Identifying General Tumor and Specific Lung Cancer Biomarkers by Transcriptomic Analysis.Biology · 2022Article
- The functions and prognostic value of Krüppel-like factors in breast cancer.Cancer cell international · 2022Article
- Bioinformatics Analysis of mRNAs and miRNAs for Identifying Potential Biomarkers in Lung Adenosquamous Carcinoma.Computational and mathematical methods in medicine · 2022Article
- A prognostic 11-DNA methylation signature for lung squamous cell carcinoma.Journal of thoracic disease · 2020Article
- Cerebral Thromboembolism after Lobectomy for Lung Cancer: Pathological Diagnosis and Mechanism of Thrombus Formation.Cancers · 2019Review
- Hepatic microcirculation and mechanisms of portal hypertension.Nature reviews. Gastroenterology & hepatology · 2019Review
- CNOT3 targets negative cell cycle regulators in non-small cell lung cancer development.Oncogene · 2019Article
- Downregulation of KLF13 through DNMT1-mediated hypermethylation promotes glioma cell proliferation and invasion.OncoTargets and therapy · 2019Article
- microRNA-32-5p targets KLF2 to promote gastric cancer by activating PI3K/AKT signaling pathway.American journal of translational research · 2019Article
Corrections and comments
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Authors and funding
13 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Kruppel-like factor 2 (KLF2) is a putative tumor suppressor gene. This study investigated its role and epigenetic mechanisms in human non-small cell lung cancer (NSCLC) in ex vivo and in vitro. A total of 47 paired NSCLC and normal tissues and six cell lines were analyzed using qRT-PCR for KLF2 expression. KLF2 methylation was assessed using the methylation specific PCR (MSP) or bisulfite sequencing PCR (BSP). Functional KLF2 region 4 (+567 to +906) was confirmed using the dual-luciferase reporter assay, while CCK-8 cell viability and flow cytometric assays were used to assess changes in cell viability, cell cycle distribution, and apoptosis after knockdown or re-expression of KLF2. Western blot was performed to analyze the effects of KLF2 shRNA on knockdown of KLF2 expression and p15 and p21 expression in cells. We found that KLF2 expression was significantly reduced in NSCLC cells and tissues via KLF2 methylation. Reduction of KLF2 expression was associated with KLF2 region 4 hypermethylation in 27 of 47 (57.45%) NSCLC tissues. Furthermore, methylation at KLF2 region 4 was significantly associated with lymph node metastasis and advanced TNM stage. Re-expression of KLF2 suppressed NSCLC cell viability, arrested cells at G0/G1 cell cycle by induction of p15 and p21 expression, and promoted apoptosis, whereas knockdown of KLF2 expression had the opposite effects on cells. Taken together, KLF2 possesses tumor suppressor functions in NSCLC and detection of KLF2 methylation should be further evaluated as a tumor or prognostic biomarker for NSCLC.
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28469808PMC5411951W2971255653What OpenQuestion holds
Registered trials
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