ArticleFrontiers in cellular neuroscience2017
Correlative STED and Atomic Force Microscopy on Live Astrocytes Reveals Plasticity of Cytoskeletal Structure and Membrane Physical Properties during Polarized Migration.
Article in Frontiers in cellular neuroscience, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 60 citations in OpenAlex.
- Tissue Dissociation Workflows Influence Reactive Astrocyte Recovery in Single-Cell Transcriptomics.Molecular neurobiology · 2026Article
- The rise of astrocytes: are they guardians or troublemakers of the brain disorder?Experimental & molecular medicine · 2026Review
- Altered Mechanical Properties of Astrocytes Lacking MLC1: Implications for the Leukodystrophy MLC.Glia · 2026Article
- Mechanics and disease of heart cells/cardiomyocytes explored through atomic force microscopy: present and future.Biophysical reviews · 2025Review
- Identification of Ultrastructural Details of the Astrocyte Process System in Nervous Tissue of the Brain Using Correlative Scanning Probe and Transmission Electron Microscopy.Doklady biological sciences : proceedings of the Academy of Sciences of the USSR, Biological sciences sections · 2023Article
- Article
- Photonics tools begin to clarify astrocyte calcium transients.Neurophotonics · 2022Review
- Microscopic Visualization of Cell-Cell Adhesion Complexes at Micro and Nanoscale.Frontiers in cell and developmental biology · 2022Review
- Correlative nanoscopy: A multimodal approach to molecular resolution.Microscopy research and technique · 2021Review
- Superresolving the kidney-a practical comparison of fluorescence nanoscopy of the glomerular filtration barrier.Analytical and bioanalytical chemistry · 2021Article
- AFM-Based Correlative Microscopy Illuminates Human Pathogens.Frontiers in cellular and infection microbiology · 2021Review
- Mechanosensation in traumatic brain injury.Neurobiology of disease · 2021Review
- Review: Cantilever-Based Sensors for High Speed Atomic Force Microscopy.Sensors (Basel, Switzerland) · 2020Review
- Review
- High-throughput, multi-parametric, and correlative fluorescence lifetime imaging.Methods and applications in fluorescence · 2020Review
- Simultaneous co-localized super-resolution fluorescence microscopy and atomic force microscopy: combined SIM and AFM platform for the life sciences.Scientific reports · 2020Article
- Nanomechanics and Histopathology as Diagnostic Tools to Characterize Freshly Removed Human Brain Tumors.International journal of nanomedicine · 2020Article
- A NanoFE simulation-based surrogate machine learning model to predict mechanical functionality of protein networks from live confocal imaging.Computational and structural biotechnology journal · 2020Article
- Bridging the gap: Super-resolution microscopy of epithelial cell junctions.Tissue barriers · 2018Review
- Cortical Actin Dynamics in Endothelial Permeability.Current topics in membranes · 2018Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
Abstract
The plasticity of the cytoskeleton architecture and membrane properties is important for the establishment of cell polarity, adhesion and migration. Here, we present a method which combines stimulated emission depletion (STED) super-resolution imaging and atomic force microscopy (AFM) to correlate cytoskeletal structural information with membrane physical properties in live astrocytes. Using STED compatible dyes for live cell imaging of the cytoskeleton, and simultaneously mapping the cell surface topology with AFM, we obtain unprecedented detail of highly organized networks of actin and microtubules in astrocytes. Combining mechanical data from AFM with optical imaging of actin and tubulin further reveals links between cytoskeleton organization and membrane properties. Using this methodology we illustrate that scratch-induced migration induces cytoskeleton remodeling. The latter is caused by a polarization of actin and microtubule elements within astroglial cell processes, which correlates strongly with changes in cell stiffness. The method opens new avenues for the dynamic probing of the membrane structural and functional plasticity of living brain cells. It is a powerful tool for providing new insights into mechanisms of cell structural remodeling during physiological or pathological processes, such as brain development or tumorigenesis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.