ArticleScientific reports2017
Multiplex detection of bacteria on an integrated centrifugal disk using bead-beating lysis and loop-mediated amplification.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 57 citations in OpenAlex.
- Intelligent microfluidic device for multiplex detection and prompt warning of upper and lower respiratory tract infections.Journal of advanced research · 2026Article
- Application of multi-channel magnetic particle immunofluorescent disc microfluidic chip for combined detection of antibodies against six common infectious diseases including visceral leishmaniasis in pastoral areas.Frontiers in cellular and infection microbiology · 2026Article
- Recent Advances in Microfluidics-Based Monitoring of Waterborne Pathogens: From Isolation to Detection.Micromachines · 2025Review
- Integrated High-Throughput Centrifugal Microfluidic Chip Device for Pathogen Detection On-Site.Biosensors · 2024Article
- Bead-based microfluidic platforms for multiplex and ultrasensitive immunoassays in clinical diagnosis and treatment.Mechanobiology in medicine · 2024Review
- Emerging Bioanalytical Devices and Platforms for Rapid Detection of Pathogens in Environmental Samples.Micromachines · 2022Review
- RETRACTED: Surface Hydrophilic Modification for Chip of Centrifugal Microfluidic Immunoassay System.Micromachines · 2022Article
- Development of a proof-of-concept microfluidic portable pathogen analysis system for water quality monitoring.The Science of the total environment · 2022Article
- Multiplexed detection of respiratory pathogens with a portable analyzer in a "raw-sample-in and answer-out" manner.Microsystems & nanoengineering · 2021Article
- Differential Markers of Bacterial and Viral Infections in Children for Point-of-Care Testing.Trends in molecular medicine · 2020Review
- Advances in point-of-care nucleic acid extraction technologies for rapid diagnosis of human and plant diseases.Biosensors & bioelectronics · 2020Review
- Electrochemical Immuno- and Aptamer-Based Assays for Bacteria: Pros and Cons over Traditional Detection Schemes.Sensors (Basel, Switzerland) · 2020Review
- Advanced "lab-on-a-chip" to detect viruses - Current challenges and future perspectives.Biosensors & bioelectronics · 2020Review
- Microfluidic-Based Nucleic Acid Amplification Systems in Microbiology.Micromachines · 2019Review
- Multiplex detection of six swine viruses on an integrated centrifugal disk using loop-mediated isothermal amplification.Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2019Article
- Impact of DNA extraction method and targeted 16S-rRNA hypervariable region on oral microbiota profiling.Scientific reports · 2018Article
- A nanofilter for fluidic devices by pillar-assisted self-assembly microparticles.Biomicrofluidics · 2018Article
- Sensitive, Real-time and Non-Intrusive Detection of Concentration and Growth of Pathogenic Bacteria using Microfluidic-Microwave Ring Resonator Biosensor.Scientific reports · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Although culture-based identification of bacteria is the gold-standard for the diagnosis of infectious diseases, it is time consuming. Recent advances in molecular diagnostics and microfluidic technologies have opened up new avenues for rapid detection of bacteria. Here, we describe a centrifugal-microfluidic chip for the detection of bacteria by integrating the cell lysis, clarification, and loop-mediated amplification (LAMP). The major advantages of this chip are as follows. Firstly, bacteria lysis was innovatively achieved by rotating a pair of magnets to generate bead-beating while the chip was kept stationary during lysis, which simplified the chip design because no additional valve was needed. Secondly, the on-chip assay time was short (within 70 min), which was competitive in emergency situations. Thirdly, results of the analysis can be interpreted by using a fluorescence detector or by the naked-eye, making it versatile in many areas, especially the resource-limited areas. The on-chip limits of detection of six types of bacteria were valued by gel electrophoresis, showing the similar results compared to the bench-top LAMP protocol. This chip can be used for rapid, sensitive, accurate and automated detection of bacteria, offering a promising alternative for simplifying the molecular diagnostics of infectious diseases.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.