ArticleNature communications2017
Automated multiplex genome-scale engineering in yeast.
Article in Nature communications, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 85 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
85 citing papers in PubMed, 198 citations in OpenAlex.
- High-throughput editing boosts the construction of next-generation microbial cell factories.Synthetic and systems biotechnology · 2026Review
- Biological parts in yeast synthetic biology: from regulatory elements to predictive design platforms.FEMS yeast research · 2026Review
- Automated Genetic Manipulation for the Construction ofChem & bio engineering · 2025Article
- Optimized Machine Learning for Autonomous Enzymatic Reaction Intensification in a Self-Driving Lab.Biotechnology and bioengineering · 2025Article
- Automated Strain Construction for Biosynthetic Pathway Screening in Yeast.ACS synthetic biology · 2025Article
- Fueling chromosomal gene diversification and artificial evolution with CRISPR.Genome biology · 2025Review
- Dual-mode CRISPRa/i for genome-scale metabolic rewiring in Escherichia coli.Nucleic acids research · 2025Article
- A generalized platform for artificial intelligence-powered autonomous enzyme engineering.Nature communications · 2025Article
- The knowledge driven DBTL cycle provides mechanistic insights while optimising dopamine production in Escherichia coli.Microbial cell factories · 2025Article
- Enhancing lipid production in plant cells through automated high-throughput genome engineering and phenotyping.The Plant cell · 2025Article
- Synthetic evolution ofmLife · 2025Review
- Advances in CRISPR-enabled genome-wide screens in yeast.FEMS yeast research · 2025Review
- Bioengineering hybrid artificial life.Frontiers in bioinformatics · 2025Review
- Engineering biology and automation-Replicability as a design principle.Engineering biology · 2024Review
- Getting the Right Clones in an Automated Manner: An Alternative to Sophisticated Colony-Picking Robotics.Bioengineering (Basel, Switzerland) · 2024Article
- Massively parallel CRISPR-assisted homologous recombination enables saturation editing of full-length endogenous genes in yeast.Science advances · 2024Article
- "Cell Disk" DNA Storage System Capable of Random Reading and Rewriting.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Automated high-throughput DNA synthesis and assembly.Heliyon · 2024Review
- Genetically Encoded Biosensor Engineering for Application in Directed Evolution.Journal of microbiology and biotechnology · 2023Review
- CRISPR-Cas Technology for Bioengineering Conventional and Non-Conventional Yeasts: Progress and New Challenges.International journal of molecular sciences · 2023Review
25 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Genome-scale engineering is indispensable in understanding and engineering microorganisms, but the current tools are mainly limited to bacterial systems. Here we report an automated platform for multiplex genome-scale engineering in Saccharomyces cerevisiae, an important eukaryotic model and widely used microbial cell factory. Standardized genetic parts encoding overexpression and knockdown mutations of >90% yeast genes are created in a single step from a full-length cDNA library. With the aid of CRISPR-Cas, these genetic parts are iteratively integrated into the repetitive genomic sequences in a modular manner using robotic automation. This system allows functional mapping and multiplex optimization on a genome scale for diverse phenotypes including cellulase expression, isobutanol production, glycerol utilization and acetic acid tolerance, and may greatly accelerate future genome-scale engineering endeavours in yeast.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.