ArticleScientific reports2017
Optical visualisation of thermogenesis in stimulated single-cell brown adipocytes.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 66 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
66 citing papers in PubMed, 1 synthesis or guideline pooled it, 110 citations in OpenAlex.
- Thermogenic Differentiation of Human Adipocyte Precursors in Culture: A Systematic Review.Cells · 2025Pooled it
- Cold-induced serum amyloid A3 amplifies cAMP-PKA signaling to drive adaptive thermogenesis in brown adipocytes.Journal of physiology and biochemistry · 2026Article
- HuR-dependent expression of RyR2 contributes to calcium-mediated thermogenesis in murine brown adipocytes.Scientific reports · 2026Article
- Non-diffusive slow heat dissipation induces high local temperature in living cells.Nature communications · 2026Article
- Inhibition of adipocyte RUNX1/2 enhances adipose tissue thermogenesis through distinct mechanisms.Nature communications · 2026Article
- Transplantation of Soluble Epoxide Hydrolase Inhibitor-Treated Human Brown Adipocytes Promotes Adipose Tissue Activation in High-Fat-Diet-Fed Nude Mice.International journal of molecular sciences · 2026Article
- Single-Nanoparticle Luminescence Nanothermometers with Enhanced Sensitivity in Physiological Temperature Range.ACS omega · 2025Article
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- Reprogramming White Fat Cells for Adipose Manipulation Transplantation (AMT) Therapy.Bio-protocol · 2025Article
- The microprotein C16orf74/MICT1 promotes thermogenesis in brown adipose tissue.The EMBO journal · 2025Article
- Creation of Genetically Modified Adipocytes for Tissue Engineering: Creatine Kinase B Overexpression Leads to Stimulated Glucose Uptake and Mitochondrial Potential Growth, but Lowered Lipid Synthesis.Life (Basel, Switzerland) · 2025Article
- Kdm2a inhibition in skeletal muscle improves metabolic flexibility in obesity.Nature metabolism · 2025Article
- Advances in malignant hyperthermia: novel insights into heat-induced CaJournal of smooth muscle research = Nihon Heikatsukin Gakkai kikanshi · 2025Review
- Genetically-encoded temperature indicators for thermal biology.Biophysics and physicobiology · 2025Article
- Hypoxia-inducible factor-1α-deficient adipose-tissue macrophages produce the heat to mediate lipolysis of white adipose tissue through uncoupling protein-1.Laboratory animal research · 2024Article
- TRPV2: a universal regulator in cellular physiology with a yet poorly defined thermosensitivity.The journal of physiological sciences : JPS · 2024Review
- Glucocorticoid modulates oxidative and thermogenic function of rat brown adipose tissue and human brown adipocytes.Journal of cellular physiology · 2024Article
- Extremely Sensitive Genetically Encoded Temperature Indicator Enabling Measurement at the Organelle Level.ACS sensors · 2024Article
- Endothelin 3/EDNRB signaling induces thermogenic differentiation of white adipose tissue.Nature communications · 2024Article
6 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
21 authors at 6 institutions in 4 countries.
Funding
Abstract
The identification of brown adipose deposits in adults has led to significant interest in targeting this metabolically active tissue for treatment of obesity and diabetes. Improved methods for the direct measurement of heat production as the signature function of brown adipocytes (BAs), particularly at the single cell level, would be of substantial benefit to these ongoing efforts. Here, we report the first application of a small molecule-type thermosensitive fluorescent dye, ERthermAC, to monitor thermogenesis in BAs derived from murine brown fat precursors and in human brown fat cells differentiated from human neck brown preadipocytes. ERthermAC accumulated in the endoplasmic reticulum of BAs and displayed a marked change in fluorescence intensity in response to adrenergic stimulation of cells, which corresponded to temperature change. ERthermAC fluorescence intensity profiles were congruent with mitochondrial depolarisation events visualised by the JC-1 probe. Moreover, the averaged fluorescence intensity changes across a population of cells correlated well with dynamic changes such as thermal power, oxygen consumption, and extracellular acidification rates. These findings suggest ERthermAC as a promising new tool for studying thermogenic function in brown adipocytes of both murine and human origins.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.