Evidence map›Paper›PMID 28469136›Full record

ArticleScientific reports2017

Identification of a nanobody specific to human pulmonary surfactant protein A.

Xian He, Shan-Mei Wang, Zhao Fang Yin, Meng-Meng Zhao, Nan Li, Feng Yu, Liu-Sheng Wang, Yang Hu, Yu-Kui Du, Shan-Shan Du and 6 more

Erratum issuedAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

16 authors.

Xian HeDepartment of Respiratory Medicine Suzhou University, School of Medicine, SuZhou, China.
Shan-Mei WangDepartment of Respiratory Medicine, Shanghai Pulmonary Hospital Tongji University, School of Medicine, Shanghai, China.
Zhao Fang YinDepartment of Respiratory Medicine Suzhou University, School of Medicine, SuZhou, China.
Meng-Meng ZhaoDepartment of Respiratory Medicine, Shanghai Pulmonary Hospital Tongji University, School of Medicine, Shanghai, China.
Nan LiDepartment of Respiratory Medicine, Shanghai Pulmonary Hospital Tongji University, School of Medicine, Shanghai, China.
Feng YuShanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai, China.
Liu-Sheng WangDepartment of Respiratory Medicine, Shanghai Pulmonary Hospital Tongji University, School of Medicine, Shanghai, China.
Yang HuDepartment of Respiratory Medicine, Shanghai Pulmonary Hospital Tongji University, School of Medicine, Shanghai, China.
Yu-Kui DuDepartment of Respiratory Medicine, Shanghai Pulmonary Hospital Tongji University, School of Medicine, Shanghai, China.
Shan-Shan DuDepartment of Respiratory Medicine, Shanghai Pulmonary Hospital Tongji University, School of Medicine, Shanghai, China.
Yan LiDepartment of Respiratory Medicine, Shanghai Pulmonary Hospital Tongji University, School of Medicine, Shanghai, China.
Ya-Ru WeiDepartment of Respiratory Medicine, Shanghai Pulmonary Hospital Tongji University, School of Medicine, Shanghai, China.
Shan-Shan ChenDepartment of Respiratory Medicine Suzhou University, School of Medicine, SuZhou, China.
Jian-Hua HeShanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai, China.
Dong WengDepartment of Respiratory Medicine, Shanghai Pulmonary Hospital Tongji University, School of Medicine, Shanghai, China. cruise00@126.com.
Hui-Ping LiDepartment of Respiratory Medicine, Shanghai Pulmonary Hospital Tongji University, School of Medicine, Shanghai, China. liw2013@126.com.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Nanobody (Nb) is a promising vector for targeted drug delivery. This study aims to identify an Nb that can specifically target the lung by binding human pulmonary surfactant protein A (SP-A). Human lung frozen tissue sections were used for 3 rounds of biospanning of our previously constructed Nb library for rat SP-A to establish a sub-library of Nb, which specifically bound human lung tissues. Phage-ELISA was performed to screen the sub-library to identify Nb4, which specifically bound human SP-A. The binding affinity Kd of Nb4 to recombinant human SP-A was 7.48 × 10

Indexed as

Drug Delivery SystemsAnimalsCell LineFemaleHumansLungMice, Inbred BALB CMice, NudePulmonary Surfactant-Associated Protein ARatsRecombinant ProteinsSingle-Domain AntibodiesTissue DistributionPulmonary Surfactant-Associated Protein ARecombinant ProteinsSingle-Domain Antibodies

Identifiers

PMID28469136
PMCPMC5431231

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.