ArticleScience translational medicine2017
Rapid and specific detection of Asian- and African-lineage Zika viruses.
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Article in Science translational medicine, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers.
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53citing papers in PubMed
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Who cites it
53 citing papers in PubMed, 104 citations in OpenAlex.
- Development of Universal Primer Sets for Zika Virus Envelope Gene Amplification and Sequencing.International journal of molecular sciences · 2026Article
- Development and validation of a LAMP assay for detection of genetically diverseFrontiers in microbiology · 2026Article
- Assessment of Hairpin-Functionalization of Gold Nanoparticles toward a Facile Detection of Zika Virus and Lineage Identification.ACS omega · 2025Article
- Bussuquara Virus: A Neglected Orthoflavivirus with Broad Distribution Across Central and South America and the Caribbean.Viruses · 2025Review
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- Advances and Challenges in SARS-CoV-2 Detection: A Review of Molecular and Serological Technologies.Diagnostics (Basel, Switzerland) · 2024Review
- Rapid and Reliable Detection of SARS-CoV-2 Using Direct RT-LAMP.Diagnostics (Basel, Switzerland) · 2022Article
- Exploring nano-enabled CRISPR-Cas-powered strategies for efficient diagnostics and treatment of infectious diseases.Journal of nanostructure in chemistry · 2022Review
- A diagnostic accuracy study comparing RNA LAMP, direct LAMP, and rapid antigen testing from nasopharyngeal swabs.Frontiers in microbiology · 2022Article
- Capillary-based reverse transcriptase loop-mediated isothermal amplification for cost-effective and rapid point-of-care COVID-19 testing.Analytica chimica acta · 2021Article
- Single-cell nucleic acid profiling in droplets (SNAPD) enables high-throughput analysis of heterogeneous cell populations.Nucleic acids research · 2021Article
- Review
- Application of Nanobiotechnology for Early Diagnosis of SARS-CoV-2 Infection in the COVID-19 Pandemic.Applied microbiology and biotechnology · 2021Review
- Article
- LAMP in Neglected Tropical Diseases: A Focus on Parasites.Diagnostics (Basel, Switzerland) · 2021Review
- Development and validation of a one-step reverse transcription loop-mediated isothermal amplification (RT-LAMP) for rapid detection of ZIKV in patient samples from Brazil.Scientific reports · 2021Article
- Saliva TwoStep for rapid detection of asymptomatic SARS-CoV-2 carriers.medRxiv : the preprint server for health sciences · 2021Article
- Loop-Mediated Isothermal Amplification in Schistosomiasis.Journal of clinical medicine · 2021Review
- Recent Advancement in SARS-CoV-2 Diagnosis, Treatment, and Vaccine Formulation: a New Paradigm of Nanotechnology in Strategic Combating of COVID-19 Pandemic.Current pharmacology reports · 2021Review
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Authors and funding
24 authors at 8 institutions in 4 countries.
Nunya ChotiwanDepartment of Microbiology, Immunology and Pathology, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO 80523, USA.ORCID 0000-0003-3214-6605
Connie D BrewsterDepartment of Microbiology, Immunology and Pathology, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO 80523, USA.
Tereza MagalhaesArthropod-borne Infectious Disease Laboratories, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO 80523, USA.
James Weger-LucarelliDepartment of Microbiology, Immunology and Pathology, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO 80523, USA.
Nisha K DuggalDivision of Vector-Borne Diseases, Centers for Disease Control and Prevention, Fort Collins, CO 80521, USA.
Claudia RückertDepartment of Microbiology, Immunology and Pathology, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO 80523, USA.
Chilinh NguyenDepartment of Microbiology, Immunology and Pathology, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO 80523, USA.
Selene M Garcia LunaDepartment of Microbiology, Immunology and Pathology, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO 80523, USA.
Joseph R FauverDepartment of Microbiology, Immunology and Pathology, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO 80523, USA.ORCID 0000-0001-8099-2991
Barb AndreDepartment of Microbiology, Immunology and Pathology, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO 80523, USA.ORCID 0000-0002-4221-1979
Meg GrayDepartment of Microbiology, Immunology and Pathology, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO 80523, USA.
William C BlackDepartment of Microbiology, Immunology and Pathology, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO 80523, USA.
Rebekah C KadingDepartment of Microbiology, Immunology and Pathology, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO 80523, USA.ORCID 0000-0002-4996-915X
Gregory D EbelDepartment of Microbiology, Immunology and Pathology, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO 80523, USA.ORCID 0000-0002-4458-9959
Guillermina KuanCentro de Salud Sócrates Flores Vivas, Ministry of Health, Managua, Nicaragua.
Angel BalmasedaLaboratorio Nacional de Virología, Centro Nacional de Diagnóstico y Referencia, Ministry of Health, Managua, Nicaragua.
Thomas JaenischSection Clinical Tropical Medicine, Department for Infectious Diseases, Heidelberg University Hospital, Heidelberg, Germany.
Ernesto T A MarquesLaboratory of Virology and Experimental Therapeutics, Centro de Pesquisas Aggeu Magalhaes, Fundacao Oswaldo Cruz, Recife-PE, Brazil.ORCID 0000-0003-3826-9358
Aaron C BraultDivision of Vector-Borne Diseases, Centers for Disease Control and Prevention, Fort Collins, CO 80521, USA.ORCID 0000-0001-5801-3021
Eva HarrisDivision of Infectious Diseases and Vaccinology, School of Public Health, University of California, Berkeley, Berkeley, CA 94720-7360, USA.
Brian D FoyDepartment of Microbiology, Immunology and Pathology, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO 80523, USA.
Sandra L QuackenbushDepartment of Microbiology, Immunology and Pathology, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO 80523, USA.ORCID 0000-0001-7941-7201
Rushika PereraDepartment of Microbiology, Immunology and Pathology, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO 80523, USA.
Joel RovnakDepartment of Microbiology, Immunology and Pathology, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO 80523, USA. joel.rovnak@colostate.edu.ORCID 0000-0003-4518-9030
Colorado State University · USCenters for Disease Control and Prevention · USBerkeley Public Health Division · USFundação Oswaldo Cruz · BRHeidelberg University · DEMinisterio de Salud · NINational Autonomous University of Nicaragua · NIUniversity of Pittsburgh · US
Funding
Project 3 - Ex vivo immune profiling of dengue viruses and vaccinesU19AI118610 · NIAID · ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI · PI WOLINSKY, STEVEN M · 2015 to 2021
$46.6MT Cell Responses Following DENV Natural Infections and Live-Attenuated Dengue Virus VaccinationP01AI106695 · NIAID · UNIVERSITY OF CALIFORNIA BERKELEY · PI Daniela Weiskopf · 2015 to 2026
$34.1MViral Immunity and VAccination (VIVA) Human Immunology Project Consortium (HIPC)U19AI168631 · NIAID · ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI · PI Ana Fernandez-Sesma, Viviana A Simon · 2022 to 2026
$14.4MQuasispecies dynamics in arbovirus persistence emergence and fitnessR01AI067380 · NIAID · UNIVERSITY OF NEW MEXICO · PI Gregory David Ebel · 2007 to 2026
$7.7MSustainable Control of Aedes aegypti and epidemic dengueU01AI088647 · NIAID · COLORADO STATE UNIVERSITY · PI BLACK, WILLIAM C. · 2010 to 2014
$3.1MEpidemiological, Clinical & Immunological Studies of Zika, dengue and chikungunyaR01AI099631 · NIAID · SUSTAINABLE SCIENCES INSTITUTE · PI BALMASEDA, ANGEL LAZARO · 2012 to 2021
$1.8MTraining in Dengue Prevention and ControlD43TW001130 · FIC · COLORADO STATE UNIVERSITY-FORT COLLINS · PI BLACK, WILLIAM C. · 1999 to 2015
$1.5MDesign and validation of a rapid assay for Zika virus in biofluids and insect vectorsR21AI129488 · NIAID · COLORADO STATE UNIVERSITY · PI ROVNAK, JOEL · 2017 to 2018
$415kZika virus urogenital tropism, pathogenesis and sexual transmissionR21AI129464 · NIAID · COLORADO STATE UNIVERSITY · PI FOY, BRIAN DAVID · 2017 to 2018
$377kFIC NIH HHS D43 TW001130NIAID NIH HHS P01 AI106695NIAID NIH HHS R01 AI067380NIAID NIH HHS R01 AI099631NIAID NIH HHS R21 AI129464NIAID NIH HHS R21 AI129488NIAID NIH HHS U01 AI088647NIAID NIH HHS U19 AI118610NIAID NIH HHS U19 AI168631
Abstract
Understanding the dynamics of Zika virus transmission and formulating rational strategies for its control require precise diagnostic tools that are also appropriate for resource-poor environments. We have developed a rapid and sensitive loop-mediated isothermal amplification (LAMP) assay that distinguishes Zika viruses of Asian and African lineages. The assay does not detect chikungunya virus or flaviviruses such as dengue, yellow fever, or West Nile viruses. The assay conditions allowed direct detection of Zika virus RNA in cultured infected cells; in mosquitoes; in virus-spiked samples of human blood, plasma, saliva, urine, and semen; and in infected patient serum, plasma, and semen samples without the need for RNA isolation or reverse transcription. The assay offers rapid, specific, sensitive, and inexpensive detection of the Asian-lineage Zika virus strain that is currently circulating in the Western hemisphere, and can also detect the African-lineage Zika virus strain using separate, specific primers.
Indexed as
AnimalsCulicidaeHumansNucleic Acid Amplification TechniquesRNA, ViralZika VirusRNA, Viral
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