ArticleMolecular biology of the cell2017
Effects of substrate stiffness and actomyosin contractility on coupling between force transmission and vinculin-paxillin recruitment at single focal adhesions.
Article in Molecular biology of the cell, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 75 papers.
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Who cites it
75 citing papers in PubMed.
- The role of ECM mechanics in cancer mechanotransduction through unraveling the molecular machinery of integrins, FAK, and YAP signaling.Cellular & molecular biology letters · 2026Review
- Hybrid Multiphoton Lithography Scaffolds for Nanoscale Mechanobiological Assessment in Microscale Bone Models.Small (Weinheim an der Bergstrasse, Germany) · 2026Article
- Shear Stress as a Driver of Retinal Müller Glia Survival and Fibrotic Reprogramming.Cell biochemistry and function · 2026Article
- MicroRNAs as Mediators of Mechanotransduction: from Fundamental Mechanisms to Therapeutic Applications.Cell biochemistry and biophysics · 2026Review
- Targeting the Akt-EphA2 axis and cell-cell adhesion enhances anoikis sensitivity in cancer cells.Scientific reports · 2026Article
- Linking molecular tension and cellular tractions: a multiscale approach to focal adhesion mechanics.Communications biology · 2026Article
- The role of extracellular matrix stiffness in regulating fibroblast behaviors and disease progression.Frontiers in immunology · 2026Review
- Alpha-actinin-1 promotes adhesion maturation and facilitates sarcomere assembly in cardiac myocytes.Molecular biology of the cell · 2025Article
- Hydrogel Viscoelasticity Modulates Cell Nascent Extracellular Matrix Deposition.Macromolecular rapid communications · 2025Article
- Differential PaxillinB dynamics at Dictyostelium cell-substrate adhesions.Biology open · 2025Article
- Attenuated Nuclear Tension Regulates Progerin-Induced Mechanosensitive Nuclear Wrinkling and Chromatin Remodeling.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Reduced cell-substrate adhesion formation promotes cell migration inMolecular biology of the cell · 2025Article
- Sustained strain applied at high rates drives dynamic tensioning in epithelial cells.Communications biology · 2025Article
- Reduced cell-substrate adhesion formation promotes cell migration inbioRxiv : the preprint server for biology · 2025Article
- Sustained Strain Applied at High Rates Drives Dynamic Tensioning in Epithelial Cells.bioRxiv : the preprint server for biology · 2025Article
- Unravelling molecular dynamics in living cells: Fluorescent protein biosensors for cell biology.Journal of microscopy · 2025Review
- Role of the cytoskeleton in cellular reprogramming: effects of biophysical and biochemical factors.Frontiers in molecular biosciences · 2025Review
- Sensing the squeeze: nuclear mechanotransduction in health and disease.Nucleus (Austin, Tex.) · 2024Review
- N-cadherin crosstalk with integrin weakens the molecular clutch in response to surface viscosity.Nature communications · 2024Article
- Geometric constraint of mechanosensing by modification of hydrogel thickness prevents stiffness-induced differentiation in bone marrow stromal cells.Journal of the Royal Society, Interface · 2024Article
15 more citing papers are in PubMed but not listed here.
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5 authors.
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Abstract
Focal adhesions (FAs) regulate force transfer between the cytoskeleton and ECM-integrin complexes. We previously showed that vinculin regulates force transmission at FAs. Vinculin residence time in FAs correlated with applied force, supporting a mechanosensitive model in which forces stabilize vinculin's active conformation to promote force transfer. In the present study, we examined the relationship between traction force and vinculin-paxillin localization to single FAs in the context of substrate stiffness and actomyosin contractility. We found that vinculin and paxillin FA area did not correlate with traction force magnitudes at single FAs, and this was consistent across different ECM stiffness and cytoskeletal tension states. However, vinculin residence time at FAs varied linearly with applied force for stiff substrates, and this was disrupted on soft substrates and after contractility inhibition. In contrast, paxillin residence time at FAs was independent of local applied force and substrate stiffness. Paxillin recruitment and residence time at FAs, however, were dependent on cytoskeletal contractility on lower substrate stiffness values. Finally, substrate stiffness and cytoskeletal contractility regulated whether vinculin and paxillin turnover dynamics are correlated to each other at single FAs. This analysis sheds new insights on the coupling among force, substrate stiffness, and FA dynamics.
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