ArticleJournal of virology1988
Mutational analysis of upstream AUG codons of poliovirus RNA.
Article in Journal of virology, 1988. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 66 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
66 citing papers in PubMed, 120 citations in OpenAlex.
- Review
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- Exploring Virus Diversity in theViruses · 2024Article
- RNA Structure in the 5' Untranslated Region of Enterovirus D68 Strains with Differing Neurovirulence Phenotypes.Viruses · 2023Article
- Picornavirus translation strategies.FEBS open bio · 2022Review
- RNA Binding Proteins as Pioneer Determinants of Infection: Protective, Proviral, or Both?Viruses · 2021Review
- Structure of the 5' Untranslated Region of Enteroviral Genomic RNA.Journal of virology · 2019Article
- Article
- An upstream protein-coding region in enteroviruses modulates virus infection in gut epithelial cells.Nature microbiology · 2019Article
- Cellular Proteins Act as Bridge Between 5' and 3' Ends of the Coxsackievirus B3 Mediating Genome Circularization During RNA Translation.Current microbiology · 2015Article
- Intergenic sequence between Arabidopsis caseinolytic protease B-cytoplasmic/heat shock protein100 and choline kinase genes functions as a heat-inducible bidirectional promoter.Plant physiology · 2014Article
- The current status of vertebrate cellular mRNA IRESs.Cold Spring Harbor perspectives in biology · 2013Review
- Conserved and variable structural elements in the 5' untranslated region of two hypoviruses from the filamentous fungus Cryphonectria parasitica.Virus research · 2011Article
- Mechanisms governing the selection of translation initiation sites on foot-and-mouth disease virus RNA.Journal of virology · 2011Article
- Polypyrimidine tract-binding protein stimulates the poliovirus IRES by modulating eIF4G binding.The EMBO journal · 2010Article
- Mechanism of initiation site selection promoted by the human rhinovirus 2 internal ribosome entry site.Journal of virology · 2010Article
- Proteinase 2Apro is essential for enterovirus replication in type I interferon-treated cells.Journal of virology · 2009Article
- Mutation of a single conserved nucleotide between the cloverleaf and internal ribosome entry site attenuates poliovirus neurovirulence.Journal of virology · 2005Article
- La autoantigen is necessary for optimal function of the poliovirus and hepatitis C virus internal ribosome entry site in vivo and in vitro.Molecular and cellular biology · 2004Article
6 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
The 5' untranslated region of poliovirus type 2 Lansing RNA consists of 744 nucleotides containing seven AUG codons which are followed by in-frame termination codons, thus forming short open reading frames (ORFs). To determine the biological significance of these small ORFs, all of the upstream AUG codons were mutated to UUG. The point mutations were introduced into an infectious poliovirus cDNA clone, and RNA transcribed in vitro from the altered cDNA was transfected into HeLa cells to recover the virus. Mutation of AUG 7 resulted in a virus (called R2-5NC-14) with a small-plaque phenotype, whereas mutation of the other six AUG codons produced virus with a wild-type plaque morphology. To determine whether the small-plaque phenotype of R2-5NC-14 was due to altered translational efficiency of the viral mRNA, we constructed chimeric mRNAs containing the 5' noncoding region of poliovirus mRNA fused to the chloramphenicol acetyltransferase (CAT) coding sequence. mRNA containing a mutated AUG 7 codon showed decreased translational efficiency in vitro. The results indicate that the upstream ORFs of poliovirus RNA are not essential for viral replication and do not act as barriers to the translation of poliovirus mRNA. AUG 7 and flanking sequences may play a positive acting role in poliovirus RNA translation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.