ArticleBMC microbiology2017
Simultaneous detection of chikungunya virus, dengue virus and human pathogenic Leptospira genomes using a multiplex TaqMan® assay.
Article in BMC microbiology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed, 30 citations in OpenAlex.
- Rapid differentiation of VLA1553 and wild-type ECSA chikungunya strains in the context of an IXCHIQ vaccination campaign.Emerging microbes & infections · 2026Article
- Evidence of Chikungunya Virus Reinfection in Two Patients With Lupus During the 2024-2025 Outbreak on Reunion Island.Open forum infectious diseases · 2026Article
- Chikungunya virus in dengue-suspected patients: Molecular evidence from the 2019 outbreak in Yangon, Myanmar.PLoS neglected tropical diseases · 2026Article
- Technical validation and implementation of a multiplex real-time PCR for differential diagnostic detection of Zika virus, yellow fever virus, dengue virus and chikungunya virus (ZYDC-PCR).BMC infectious diseases · 2026Article
- Development and validation of a sample-to-result real time multiplex RT-PCR test for human African trypanosomiasis and arboviral febrile illnesses in the Democratic Republic of the Congo.BMC infectious diseases · 2026Observational
- Laboratory Diagnosis of Leptospirosis.Methods in molecular biology (Clifton, N.J.) · 2026Review
- Technical validation of a multiplex real-time PCR for combined detection of Rift Valley fever, chikungunya, Zika and dengue viruses.Journal of virological methods · 2025Article
- Fatal Adverse Event After VLA1553 Chikungunya Vaccination in an Elderly Patient: A Case Report From Reunion Island.Open forum infectious diseases · 2025Article
- Pathogenicity and virulence of chikungunya virus.Virulence · 2024Review
- A Comparative Study of Human Leptospirosis between Mayotte and Reunion Islands Highlights Distinct Clinical and Microbial Features Arising from Distinct Inter-Island Bacterial Ecology.The American journal of tropical medicine and hygiene · 2024Article
- Investigation of Dengue Infection in Asymptomatic Individuals during a Recent Outbreak in La Réunion.Viruses · 2023Article
- C-reactive protein: An easy marker for early differentiation between leptospirosis and dengue fever in endemic area.PloS one · 2023Article
- Design and Analysis of a Single System of Impedimetric Biosensors for the Detection of Mosquito-Borne Viruses.Biosensors · 2021Article
- Ending the Neglect of Treatable Bacterial Zoonoses Responsible for Non-Malaria Fevers.The Yale journal of biology and medicine · 2021Review
- Urban Ecology of Arboviral Mosquito Vectors Along the Kenyan Coast.Journal of medical entomology · 2021Article
- In vitro shared transcriptomic responses of Aedes aegypti to arboviral infections: example of dengue and Rift Valley fever viruses.Parasites & vectors · 2020Article
- Integrated Arbovirus Surveillance Improves the Detection Onset of Zika Virus in Panama.The American journal of tropical medicine and hygiene · 2020Article
- Article
- The monocytosis during human leptospirosis is associated with modest immune cell activation states.Medical microbiology and immunology · 2019Article
- Dengue virus: a review on advances in detection and trends - from conventional methods to novel biosensors.Mikrochimica acta · 2019Review
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundIn 2005-2006 a major epidemics of Chikungunya disease occurred in South-West Indian Ocean islands. In Reunion Island, the magnitude of Chikungunya infection related symptoms was high and with over 38% of serological prevalence in the population. This epidemics illustrated the potential threat of emerging arboviral diseases for inhabitants of Reunion Island and elsewhere since vectors are worldwide distributed. A sentinel surveillance network was set-up to detect emerging pathogens associated with fever over 38 °C and in the absence of known etiologic causes. Leptospirosis is caused by a pathogenic spirochete of the Leptospira genus and is an endemic and recurrent seasonal disease of great concern in Reunion Island. To accurately diagnose potentially infected patients and to advise Health authorities on the presence of emerging pathogens, a rapid diagnostic test was needed that could differentiate between these 3 pathogens.
methodsA one-step multiplex real-time PCR assay was developed that can simultaneously detect RNA of Chikungunya and Dengue viruses and leptospiral DNA with good performance for a routine diagnostic use.
resultsSimplex protocols already published were used with key modifications to implement a triplex assay which was set-up with a small reaction volume to improve cost efficiency.
conclusionsThis approach has enabled greater diagnostic capacity in our laboratory. We established a multiplex approach validated and valuable for cost savings, and with the concurrent detection of 3 pathogens of public health concern.
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