Evidence map›Paper›PMID 28468234›Full record

ArticleToxins2017

Development of an Immunochromatographic Strip Test for the Rapid Detection of Alternariol Monomethyl Ether in Fruit.

Yan Man, Gang Liang, Fuchao Jia, An Li, Hailong Fu, Meng Wang, Ligang Pan

Open access · goldAbstract read
In one paragraph

Article in Toxins, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
1.2field-weighted citation impact, top 23% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 17 citations in OpenAlex.

  1. Article
  2. Occurrence and Dietary Risk Assessment ofFoods (Basel, Switzerland) · 2026
    Article
  3. Article
  4. Article
  5. Simultaneous Rapid Detection of Aflatoxin BFoods (Basel, Switzerland) · 2021
    Article
  6. Article
  7. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Yan ManBeijing Research Center for Agricultural Standards and Testing, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China. manyan3669@163.com.
Gang LiangBeijing Research Center for Agricultural Standards and Testing, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China. liangg@brcast.org.cn.
Fuchao JiaSchool of Science, Shandong University of Technology, Zibo 255000, Shandong, China. jiafuchao@sdut.edu.cn.
An LiBeijing Research Center for Agricultural Standards and Testing, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China. lia@brcast.org.cn.
Hailong FuBeijing Research Center for Agricultural Standards and Testing, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China. fuhl@brcast.org.cn.
Meng WangBeijing Research Center for Agricultural Standards and Testing, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China. wangm@brcast.org.cn.
Ligang PanBeijing Research Center for Agricultural Standards and Testing, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China. panlg@brcast.org.cn.
Beijing Municipal Ecological and Environmental Monitoring Center · CNShandong University of Technology · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

A rapid, portable, and semi-quantitative immunochromatographic strip was developed for rapid and visual detection of alternariol monomethyl ether (AME). For this purpose, the anti-AME monoclonal antibody (mAb) was prepared and identified. AME coupled to bovine serum albumin (BSA) via methyl 4-bromobutanoate was prepared as immunogen. The recoveries of AME in spiked cherry and orange fruits determined by competitive ELISA were 86.1% and 80.7%, respectively. A colloidal gold nanoparticle (CGN) and CGNs-mAb conjugate were synthesized, and on this basis, a competitive colloidal gold immunochromatographic strip was developed and applied to the detection of AME toxin in fruit samples. The intensity of red density of the test line (T line) is inversely proportional to AME concentration in the range 0.1-10 ng/mL. The visual limit of detection (LOD) of AME was found to be about 10 ng/mL. The semi-quantitative detection can be completed in 10 min. Moreover, the immunochromatographic strip has lower cross-reactivity with AME analogues, and it has a good stability performance (following 3 months of storage). Hence, the colloidal gold immunochromatographic strip could be used as a semi-quantitative tool for the on-site, rapid, and visual detection of AME in fruit.

Indexed as

AnimalsAntibodies, MonoclonalChromatography, AffinityCitrus sinensisFood ContaminationFruitGold ColloidImmunoconjugatesLactonesLimit of DetectionMetal NanoparticlesMice, Inbred BALB COvalbuminPrunus aviumSerum Albumin, Bovinealternariol monomethyl etherAntibodies, MonoclonalGold ColloidImmunoconjugatesLactonesOvalbuminSerum Albumin, Bovinealternariol monomethyl ether (AME)colloidal gold nanoparticlecompetitive ELISAimmunochromatographic strip testmonoclonal antibody

Identifiers

PMID28468234
PMCPMC5450700
OpenAlexW2611705934

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.