ArticleNature communications2017
Pointwise error estimates in localization microscopy.
Article in Nature communications, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
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- Multicolor Quantum Dot Tracking Uncovers Phenotypic Rescue of DAT A559V Aberrant Diffusion Upon D2R Antagonism.ACS chemical neuroscience · 2026Article
- Optimization of the genetic code expansion technology for intracellular labelling and single-molecule tracking of proteins in genomically re-codedRSC chemical biology · 2026Article
- A complex between IF2 and NusA suggests early coupling of transcription-translation.Nature communications · 2025Article
- Dynamic binding of the bacterial chaperone Trigger factor to translating ribosomes inProceedings of the National Academy of Sciences of the United States of America · 2025Article
- The Escherichia coli chromosome moves to the replisome.Nature communications · 2024Article
- Single-molecule tracking to determine the abundances and stoichiometries of freely-diffusing protein complexes in living cells: Past applications and future prospects.The Journal of chemical physics · 2023Review
- ExTrack characterizes transition kinetics and diffusion in noisy single-particle tracks.The Journal of cell biology · 2023Article
- Error-Correction Method for High-Throughput Sizing of Nanoscale Vesicles with Single-Molecule Localization Microscopy.The journal of physical chemistry. B · 2023Article
- Spatiotemporal kinetics of the SRP pathway in liveProceedings of the National Academy of Sciences of the United States of America · 2022Article
- Article
- Direct measurements of mRNA translation kinetics in living cells.Nature communications · 2022Article
- SMAUG: Analyzing single-molecule tracks with nonparametric Bayesian statistics.Methods (San Diego, Calif.) · 2021Article
- Bayesian Inference: The Comprehensive Approach to Analyzing Single-Molecule Experiments.Annual review of biophysics · 2021Review
- Real-time measurements of aminoglycoside effects on protein synthesis in live cells.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Blind Deconvolution Based on Compressed Sensing with bi-International journal of environmental research and public health · 2021Article
- NOBIAS: Analyzing anomalous diffusion in single-molecule tracks with nonparametric Bayesian inference.Frontiers in bioinformatics · 2021Article
- Quantifying accuracy and heterogeneity in single-molecule super-resolution microscopy.Nature communications · 2020Article
- Variational Algorithms for Analyzing Noisy Multistate Diffusion Trajectories.Biophysical journal · 2018Article
- tRNA tracking for direct measurements of protein synthesis kinetics in live cells.Nature chemical biology · 2018Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pointwise localization of individual fluorophores is a critical step in super-resolution localization microscopy and single particle tracking. Although the methods are limited by the localization errors of individual fluorophores, the pointwise localization precision has so far been estimated using theoretical best case approximations that disregard, for example, motion blur, defocus effects and variations in fluorescence intensity. Here, we show that pointwise localization precision can be accurately estimated directly from imaging data using the Bayesian posterior density constrained by simple microscope properties. We further demonstrate that the estimated localization precision can be used to improve downstream quantitative analysis, such as estimation of diffusion constants and detection of changes in molecular motion patterns. Finally, the quality of actual point localizations in live cell super-resolution microscopy can be improved beyond the information theoretic lower bound for localization errors in individual images, by modelling the movement of fluorophores and accounting for their pointwise localization uncertainty.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.