ArticleWorld journal of microbiology & biotechnology2017
A new and efficient approach for construction of uridine/uracil auxotrophic mutants in the filamentous fungus Aspergillus oryzae using Agrobacterium tumefaciens-mediated transformation.
Article in World journal of microbiology & biotechnology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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30 citing papers in PubMed, 47 citations in OpenAlex.
- Development of a novel transformation system using zhongshengmycin inSynthetic and systems biotechnology · 2026Article
- Aokap9 gene knockout contributes to kojic acid synthesis in Aspergillus oryzae.Microbial cell factories · 2025Article
- Enhanced production of ergothioneine in Aspergillus oryzae.Applied microbiology and biotechnology · 2025Article
- Expression pattern, subcellular localization ofApplied and environmental microbiology · 2025Article
- The Zn(II)IMA fungus · 2025Article
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- Screening of pathogenicity-deficient Penicillium italicum mutants established by Agrobacterium tumefaciens-mediated transformation.Molecular genetics and genomics : MGG · 2024Article
- Sub-genomic RNAi-assisted strain evolution of filamentous fungi for enhanced protein production.Applied and environmental microbiology · 2024Article
- Construction of a NewJournal of microbiology and biotechnology · 2024Article
- Synthetic Biology Tools for EngineeringJournal of fungi (Basel, Switzerland) · 2024Review
- Functional role of a novel zinc finger protein, AoZFA, in growth and kojic acid synthesis inApplied and environmental microbiology · 2023Article
- An efficient Agrobacterium-mediated system based on the pyrG auxotrophic marker for recombinant expression in the filamentous fungus Penicillium rubens.Biotechnology letters · 2023Article
- Large-Scale Production of Anti-RNase A VHH Expressed inCurrent issues in molecular biology · 2023Article
- A newly constructed Agrobacterium-mediated transformation system based on the hisB auxotrophic marker for genetic manipulation in Aspergillus niger.Archives of microbiology · 2023Article
- The expression pattern, subcellular localization and function of three sterol 14α-demethylases inFrontiers in genetics · 2023Article
- Overexpression of a novel gene Aokap2 affects the growth and kojic acid production in Aspergillus oryzae.Molecular biology reports · 2022Article
- Identification of Six Thiolases and Their Effects on Fatty Acid and Ergosterol Biosynthesis inApplied and environmental microbiology · 2022Article
- Article
- A highly efficient identification of mutants generated by CRISPR/Cas9 using the non‑functional DsRed assisted selection in Aspergillus oryzae.World journal of microbiology & biotechnology · 2021Article
- The ancient koji mold (Aspergillus oryzae) as a modern biotechnological tool.Bioresources and bioprocessing · 2021Review
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Authors and funding
9 authors at 2 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Aspergillus oryzae is a filamentous fungus widely used in food industry and as a microbial cell factory for recombinant protein production. Due to the inherent resistance of A. oryzae to common antifungal compounds, genetic transformation of this mold usually requires auxotrophic mutants. In this study, we show that Agrobacterium tumefaciens-mediated transformation (ATMT) method is very efficient for deletion of the pyrG gene in different Aspergillus oryzae wild-type strains to generate uridine/uracil auxotrophic mutants. Our data indicated that all the obtained uridine/uracil auxotrophic transformants, which are 5- fluoroorotic acid (5-FOA) resistant, exist as the pyrG deletion mutants. Using these auxotrophic mutants and the pyrG selectable marker for genetic transformation via A. tumefaciens, we could get about 1060 transformants per 10
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