Evidence map›Paper›PMID 28466275›Full record

ArticleJournal of fluorescence2017

Development of Fluorescence-Linked Immunosorbent Assay for Icariin.

Jinjun Cheng, Yan Zhao, Meiling Zhang, Yue Zhang, Xiaoman Liu, Xin Yan, Yongzhi Wang, Ziwei Sun, Qingguo Wang, Huihua Qu

Abstract read
PubMed Publisher
In one paragraph

Article in Journal of fluorescence, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.3field-weighted citation impact, top 33% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 3 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 1 institution in 1 country.

Jinjun ChengSchool of Basic Medical Sciences, Beijing University of Chinese Medicine, 11 Beisanhuandong Road, Chaoyang District, Beijing, 100029, China.
Yan ZhaoSchool of Basic Medical Sciences, Beijing University of Chinese Medicine, 11 Beisanhuandong Road, Chaoyang District, Beijing, 100029, China.
Meiling ZhangSchool of Chinese Materia Medica, Beijing University of Chinese Medicine, 11 Beisanhuandong Road, Chaoyang District, Beijing, 100029, China.
Yue ZhangSchool of Chinese Materia Medica, Beijing University of Chinese Medicine, 11 Beisanhuandong Road, Chaoyang District, Beijing, 100029, China.
Xiaoman LiuSchool of Basic Medical Sciences, Beijing University of Chinese Medicine, 11 Beisanhuandong Road, Chaoyang District, Beijing, 100029, China.
Xin YanSchool of Chinese Materia Medica, Beijing University of Chinese Medicine, 11 Beisanhuandong Road, Chaoyang District, Beijing, 100029, China.
Yongzhi WangSchool of Chinese Materia Medica, Beijing University of Chinese Medicine, 11 Beisanhuandong Road, Chaoyang District, Beijing, 100029, China.
Ziwei SunSchool of Basic Medical Sciences, Beijing University of Chinese Medicine, 11 Beisanhuandong Road, Chaoyang District, Beijing, 100029, China.
Qingguo WangSchool of Basic Medical Sciences, Beijing University of Chinese Medicine, 11 Beisanhuandong Road, Chaoyang District, Beijing, 100029, China.
Huihua QuBeijing Institute of Traditional Chinese Medicine, Beijing University of Chinese Medicine, 11 Beisanhuandong Road, Chaoyang District, Beijing, 100029, China. quhuihuadr@163.com.
Beijing University of Chinese Medicine · CN

Funding

National Natural Science Foundation of China 81274043National Natural Science Foundation of China 81473338National Natural Science Foundation of China 81573573
6 · The paper itself

Abstract

We have been looking for a faster and simpler method for traditional Chinese medicine and natural product assay. In this study, we developed a fluorescent immunoassay approach to detect icariin (ICA) using a fluorescently labelled monoclonal antibody. The ICA-specific antibody was purified by the caprylic acid-ammonium sulphate method and then labelled with rhodamine B isothiocyanate (RBITC). Subsequently, an indirect competitive fluorescence-linked immunosorbent assay (icFLISA) was developed to detect ICA using RBITC-labelled anti-ICA MAbs. The RBITC-labelled monoclonal antibody was highly specific for ICA. The fluorescence assay demonstrated an effective ICA measurement range of 1.28 ng/mL to 20 μg/mL (R

Indexed as

FluorescenceAntibodies, MonoclonalEnzyme-Linked Immunosorbent AssayFlavonoidsFluorescent Antibody TechniqueFluorescent DyesImmunosorbentsLimit of DetectionRhodaminesAntibodies, MonoclonalFlavonoidsFluorescent DyesicariinImmunosorbentsrhodamine isothiocyanateRhodaminesEnzyme-linked immunosorbent assayFluorescence-linked immunosorbent assayFluorescently labelled monoclonal antibodyIcariinRhodamine B isothiocyanate

Identifiers

PMID28466275
OpenAlexW3097946567

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.