ArticleScientific reports2017
Paper-based RNA detection and multiplexed analysis for Ebola virus diagnostics.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
45 citing papers in PubMed, 129 citations in OpenAlex.
- Revolutionizing Healthcare With Paper-Based Nucleic Acid Testing.Exploration (Beijing, China) · 2026Review
- Trends of nucleic acid - based point-of-care diagnostics for infectious diseases.Journal of biological engineering · 2026Review
- Isothermal real-time RT-RPA for Machupo virus detection: Field-adaptable sensitivity comparable with laboratory PCR.PloS one · 2026Article
- Article
- Advances in Nucleic Acid Assays for Infectious Disease: The Role of Microfluidic Technology.Molecules (Basel, Switzerland) · 2024Review
- Microfluidic Device-Based Virus Detection and Quantification in Future Diagnostic Research: Lessons from the COVID-19 Pandemic.Biosensors · 2023Review
- An Integrated Paper Microfluidic Device Based on Isothermal Amplification for Simple Sample-to-Answer Detection ofApplied and environmental microbiology · 2023Article
- Promise and perils of paper-based point-of-care nucleic acid detection for endemic and pandemic pathogens.Lab on a chip · 2023Review
- Review
- Nanozyme-strip for rapid and ultrasensitive nucleic acid detection of SARS-CoV-2.Biosensors & bioelectronics · 2022Article
- Review
- A Systematic Review on Suitability of Molecular Techniques for Diagnosis and Research into Infectious Diseases of Concern in Resource-Limited Settings.Current issues in molecular biology · 2022Review
- Optimizing Public Health Preparedness for Highly Infectious Diseases in Central Vietnam.Diagnostics (Basel, Switzerland) · 2022Article
- Glycosylated gold nanoparticles in point of care diagnostics: from aggregation to lateral flow.Chemical Society reviews · 2022Review
- Review
- Quantitative isothermal amplification on paper membranes using amplification nucleation site analysis.Lab on a chip · 2022Article
- Toehold switch based biosensors for sensing the highly trafficked rosewoodSynthetic and systems biotechnology · 2022Article
- Paper-Based Biosensors for COVID-19: A Review of Innovative Tools for Controlling the Pandemic.ACS omega · 2021Review
- Micro and Nanoscale Technologies for Diagnosis of Viral Infections.Small (Weinheim an der Bergstrasse, Germany) · 2021Review
- [Applications of microfluidic paper-based chips in environmental analysis and detection].Se pu = Chinese journal of chromatography · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The most performing techniques enabling early diagnosis of infectious diseases rely on nucleic acid detection. Today, because of their high technicality and cost, nucleic acid amplification tests (NAAT) are of benefit only to a small fraction of developing countries population. By reducing costs, simplifying procedures and enabling multiplexing, paper microfluidics has the potential to considerably facilitate their accessibility. However, most of the studies performed in this area have not quit the lab. This letter brings NAAT on paper closer to the field, by using clinical samples and operating in a resource-limited setting. We first performed isothermal reverse transcription and Recombinase Polymerase Amplification (RT-RPA) of synthetic Ribonucleic Acid (RNA) of Ebola virus using paper microfluidics devices. We further applied this method in Guinea to detect the presence of Ebola virus in human sample RNA extracts, with minimal facilities (carry-on detection device and freeze-dried reagents on paper). RT-RPA results were available in few minutes and demonstrate a sensitivity of 90.0% compared to the gold-standard RT-PCR on a set of 43 patient samples. Furthermore, the realization of a nine-spot multilayered device achieving the parallel detection of three distinct RNA sequences opens a route toward the detection of multiple viral strains or pathogens.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.