ArticleScientific reports2017
Comparative authentication of Hypericum perforatum herbal products using DNA metabarcoding, TLC and HPLC-MS.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed.
- Multi-Platform Metabolomics for Fraud Detection in Spices and Botanical Food Supplements: From Fingerprint Acquisition to Decision-Grade Evidence.Molecules (Basel, Switzerland) · 2026Review
- Proteogenomic investigation of plant constituents in herbal beverages.NPJ science of food · 2026Article
- Phytochemical Profile and Biological Activities of Rtanj'sPlants (Basel, Switzerland) · 2025Article
- Development of a mitochondrial mini-barcode and its application in metabarcoding for identification of leech in traditional Chinese medicine.Scientific reports · 2025Article
- Integrating DNA Barcoding Within an Orthogonal Approach for Herbal Product Authentication: A Narrative Review.Phytochemical analysis : PCA · 2025Review
- DNA metabarcoding unveils authenticity and adulteration in commercial Chinese polyherbal preparations: Renshen Jianpi Wan as a critical case study.Frontiers in pharmacology · 2025Article
- Phytochemical Analysis, Antioxidant Potential and Antibacterial Activities of Different Anatomical Parts ofTheScientificWorldJournal · 2024Article
- Authentication of milk thistle commercial products using UHPLC-QTOF-ESI + MS metabolomics and DNA metabarcoding.BMC complementary medicine and therapies · 2023Article
- Check Your Shopping Cart: DNA Barcoding and Mini-Barcoding for Food Authentication.Foods (Basel, Switzerland) · 2023Article
- Detecting and Profiling of Milk Thistle Metabolites in Food Supplements: A Safety-Oriented Approach by Advanced Analytics.Metabolites · 2023Review
- Quality Consistency of Herbal Products: Chemical Evaluation.Progress in the chemistry of organic natural products · 2023Article
- Phytochemical Profiles and Biological Studies of Selected Botanical Dietary Supplements Used in the United States.Progress in the chemistry of organic natural products · 2023Article
- A combined approach of DNA metabarcoding collectively enhances the detection efficiency of medicinal plants in single and polyherbal formulations.Frontiers in plant science · 2023Article
- Identification of compounds from chufa (Food science & nutrition · 2023Article
- Next-generation sequencing and targeted quantitative real-time polymerase chain reaction for detection ofAnnals of translational medicine · 2022Article
- Article
- Advancements and future prospective of DNA barcodes in the herbal drug industry.Frontiers in pharmacology · 2022Review
- Development and Validation of a Tool to Improve Community Pharmacists' Surveillance Role in the Safe Dispensing of Herbal Supplements.Frontiers in pharmacology · 2022Article
- DNA-Based Herbal Teas' Authentication: An ITS2 andPlants (Basel, Switzerland) · 2021Article
- Antibacterial Activity of Medicinal Plants and Their Constituents in the Context of Skin and Wound Infections, Considering European Legislation and Folk Medicine-A Review.International journal of molecular sciences · 2021Review
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Authors and funding
9 authors.
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Abstract
Many herbal products have a long history of use, but there are increasing concerns over product efficacy, safety and quality in the wake of recent cases exposing discrepancies between labeling and constituents. When it comes to St. John's wort (Hypericum perforatum L.) herbal products, there is limited oversight, frequent off-label use and insufficient monitoring of adverse drug reactions. In this study, we use amplicon metabarcoding (AMB) to authenticate 78 H. perforatum herbal products and evaluate its ability to detect substitution compared to standard methods using thin-layer chromatography (TLC) and high performance liquid chromatography coupled with mass spectrometry (HPLC-MS). Hypericum perforatum was detected in 68% of the products using AMB. Furthermore, AMB detected incongruence between constituent species and those listed on the label in all products. Neither TLC nor HPLC-MS could be used to unambiguously identify H. perforatum. They are accurate methods for authenticating presence of the target compounds, but have limited efficiency in detecting infrageneric substitution and do not yield any information on other plant ingredients in the products. Random post-marketing AMB of herbal products by regulatory agencies could raise awareness among consumers of substitution and would provide an incentive to manufacturers to increase quality control from raw ingredients to commercialized products.
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