ArticleCryobiology2017
3-D printing provides a novel approach for standardization and reproducibility of freezing devices.
Article in Cryobiology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed.
- An open-hardware platform for conservation of amphibian genetic resources based on gelatin capsules.iScience · 2026Article
- Exploring pathways toward open-hardware ecosystems to safeguard genetic resources for biomedical research communities using aquatic model species.Journal of experimental zoology. Part B, Molecular and developmental evolution · 2024Review
- Transitioning from a research protocol to a scalable applied pathway for Xenopus laevis sperm cryopreservation at a national stock center: The effect of cryoprotectants.Journal of experimental zoology. Part B, Molecular and developmental evolution · 2024Article
- A comprehensive review and update on human fertility cryopreservation methods and tools.Frontiers in veterinary science · 2023Review
- Design, alpha testing, and beta testing of a 3-D printed open-hardware portable cryopreservation device for aquatic species.Journal of applied aquaculture · 2023Article
- The emerging role of open technologies for community-based improvement of cryopreservation and quality management for repository development in aquatic species.Animal reproduction science · 2022Review
- Cryopreservation ofAnimals : an open access journal from MDPI · 2022Article
- Is catalase an effective additive to alleviate oxidative stress during cryopreservation of zebrafish sperm at the repository level?Cryobiology · 2022Article
- An open hardware 3-D printed device for measuring tensile properties of thermoplastic filament polymers at cryogenic temperatures.Cryogenics · 2022Article
- Development of an open hardware 3-D printed conveyor device for continuous cryopreservation of non-batched samples.Aquacultural engineering · 2021Article
- A 3D Printed Vitrification Device for Storage in Cryopreservation Vials.Applied sciences (Basel, Switzerland) · 2021Article
- An Impedimetric Sensing Probe Based on Printed Circuit Board Technology for Monitoring in Cryobiology Applications.Journal of the Electrochemical Society · 2021Article
- A generalized approach for sperm cryopreservation in the genusJournal of the World Aquaculture Society · 2021Article
- 3-D Printed Customizable Vitrification Devices for Preservation of Genetic Resources of Aquatic Species.Aquacultural engineering · 2020Article
- Human sperm vitrification: the state of the art.Reproductive biology and endocrinology : RB&E · 2020Review
- On-Site Capabilities of a Mobile Laboratory for Aquatic Germplasm Cryopreservation.North American journal of aquaculture · 2019Article
- Three-dimensional printing can provide customizable probes for sensing and monitoring in cryobiology applications.Cryobiology · 2019Article
- Article
- Workshop report: Cryopreservation of aquatic biomedical models.Cryobiology · 2019Article
- Design and Cost Analysis of a Self-contained Mobile Laboratory for Commercial-scale Aquatic Species Cryopreservation.Journal of the World Aquaculture Society · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
Abstract
Cryopreservation has become an important and accepted tool for long-term germplasm conservation of animals and plants. To protect genetic resources, repositories have been developed with national and international cooperation. For a repository to be effective, the genetic material submitted must be of good quality and comparable to other submissions. However, due to a variety of reasons, including constraints in knowledge and available resources, cryopreservation methods for aquatic species vary widely across user groups which reduces reproducibility and weakens quality control. Herein we describe a standardizable freezing device produced using 3-dimensional (3-D) printing and introduce the concept of network sharing to achieve aggregate high-throughput cryopreservation for aquatic species. The objectives were to: 1) adapt widely available polystyrene foam products that would be inexpensive, portable, and provide adequate work space; 2) develop a design suitable for 3-D printing that could provide multiple configurations, be inexpensive, and easy to use, and 3) evaluate various configurations to attain freezing rates suitable for various common cryopreservation containers. Through this approach, identical components can be accessed globally, and we demonstrated that 3-D printers can be used to fabricate parts for standardizable freezing devices yielding relevant and reproducible cooling rates across users. With standardized devices for freezing, methods and samples can harmonize into an aggregated high-throughput pathway not currently available for aquatic species repository development.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.