Evidence map›Paper›PMID 28464817›Full record

Trial reportBMC medical genomics2017

Post-operative atrial fibrillation examined using whole-genome RNA sequencing in human left atrial tissue.

Martin I Sigurdsson, Louis Saddic, Mahyar Heydarpour, Tzuu-Wang Chang, Prem Shekar, Sary Aranki, Gregory S Couper, Stanton K Shernan, Jochen D Muehlschlegel, Simon C Body

Registry-linked trialOpen access · goldAbstract readClinical Trial
PubMed Full text at PMC Publisher
In one paragraph

Trial report in BMC medical genomics, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT00833313 (Gene Expression Profiling in Subjects With Postoperative Atrial Fibrillation After Cardiac Surgery), which is not on this map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
1.8field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

NCT00833313 unknown statusnot on this map

Gene Expression Profiling in Subjects With Postoperative Atrial Fibrillation After Cardiac Surgery (GENEXPRO Surg)

TypeobservationalSponsorBrigham and Women's HospitalRan2008 to 2025Enrolled350ConditionsAtrial FibrillationArmsAtrial biopsy
3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 21 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
  4. Review
  5. Article
  6. Article
  7. Review
  8. Inhibition of NaBasic research in cardiology · 2020
    Article
  9. Article
  10. Review
  11. Proteomics and transcriptomics in atrial fibrillation.Herzschrittmachertherapie & Elektrophysiologie · 2018
    Review
  12. Using Gene Expression to Annotate Cardiovascular GWAS Loci.Frontiers in cardiovascular medicine · 2018
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 1 country.

Martin I SigurdssonDepartment of Anesthesiology, Perioperative and Pain Medicine, Brigham and Women's Hospital/Harvard Medical School, 75 Francis Street, Boston, MA, 02115, USA. martiningi@gmail.com.
Louis SaddicDepartment of Anesthesiology, Perioperative and Pain Medicine, Brigham and Women's Hospital/Harvard Medical School, 75 Francis Street, Boston, MA, 02115, USA.
Mahyar HeydarpourDepartment of Anesthesiology, Perioperative and Pain Medicine, Brigham and Women's Hospital/Harvard Medical School, 75 Francis Street, Boston, MA, 02115, USA.
Tzuu-Wang ChangDepartment of Anesthesiology, Perioperative and Pain Medicine, Brigham and Women's Hospital/Harvard Medical School, 75 Francis Street, Boston, MA, 02115, USA.
Prem ShekarDivision of Cardiac Surgery, Department of Surgery, Brigham and Women's Hospital/Harvard Medical School, Boston, MA, USA.
Sary ArankiDivision of Cardiac Surgery, Department of Surgery, Brigham and Women's Hospital/Harvard Medical School, Boston, MA, USA.
Gregory S CouperDivision of Cardiac Surgery, Department of Surgery, Brigham and Women's Hospital/Harvard Medical School, Boston, MA, USA.
Stanton K ShernanDepartment of Anesthesiology, Perioperative and Pain Medicine, Brigham and Women's Hospital/Harvard Medical School, 75 Francis Street, Boston, MA, 02115, USA.
Jochen D MuehlschlegelDepartment of Anesthesiology, Perioperative and Pain Medicine, Brigham and Women's Hospital/Harvard Medical School, 75 Francis Street, Boston, MA, 02115, USA.
Simon C BodyDepartment of Anesthesiology, Perioperative and Pain Medicine, Brigham and Women's Hospital/Harvard Medical School, 75 Francis Street, Boston, MA, 02115, USA.
Harvard University · USBrigham and Women's Hospital · US

Funding

Identification of Genetic and Molecular Mechanisms of Atrial FibrillationR01HL098601 · NHLBI · BRIGHAM AND WOMEN'S HOSPITAL · PI BODY, SIMON C · 2010 to 2014
$2.2M
Genetic Etiology of Bicuspid Aortic Valve DiseaseR01HL114823 · NHLBI · BRIGHAM AND WOMEN'S HOSPITAL · PI BODY, SIMON C · 2012 to 2016
$2.1M
NHLBI NIH HHS R01 HL098601NHLBI NIH HHS R01 HL114823
6 · The paper itself

Abstract

backgroundBoth ambulatory atrial fibrillation (AF) and post-operative AF (poAF) are associated with substantial morbidity and mortality. Analyzing the tissue-specific gene expression in the left atrium (LA) can identify novel genes associated with AF and further the understanding of the mechanism by which previously identified genetic variants associated with AF mediate their effects.

methodsLA free wall samples were obtained intraoperatively immediately prior to mitral valve surgery in 62 Caucasian individuals. Gene expression was quantified on mRNA harvested from these samples using RNA sequencing. An expression quantitative trait loci (eQTL) analysis was performed, comparing gene expression between different genotypes of 1.0 million genetic markers, emphasizing genomic regions and genes associated with AF.

resultsComparison of whole-genome expression between patients who later developed poAF and those who did not identified 23 differentially expressed genes. These included genes associated with the resting membrane potential modified by potassium currents, as well as genes within Wnt signaling and cyclic GMP metabolism. The eQTL analysis identified 16,139 cis eQTL relationships in the LA, including several involving genes and single nucleotide polymorphisms (SNPs) linked to AF. A previous relationship between rs3744029 and MYOZ1 expression was confirmed, and a novel relationship between rs6795970 and the expression of the SCN10A gene was identified.

conclusionsThe current study is the first analysis of the human LA expression landscape using high-throughput RNA sequencing. Several novel genes and variants likely involved in AF pathogenesis were identified, thus furthering the understanding of how variants associated with AF mediate their effects via altered gene expression.

trial registrationClinicalTrials.gov ID: NCT00833313 , registered 5. January 2009.

Indexed as

Gene Expression RegulationGenetic Predisposition to DiseasePolymorphism, Single NucleotideQuantitative Trait LociAgedAtrial FibrillationCarrier ProteinsCyclic GMPFemaleGenetic Association StudiesHeart AtriaHigh-Throughput Nucleotide SequencingHumansMaleMembrane PotentialsMiddle AgedCarrier ProteinsCyclic GMPMuscle ProteinsMYOZ1 protein, humanNAV1.8 Voltage-Gated Sodium ChannelSCN10A protein, humanAtrial fibrillationeQTLExpression quantitative trait lociMitral valve surgery

Identifiers

PMID28464817
PMCPMC5414158
OpenAlexW2611465898

What OpenQuestion holds

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LicenceCC BY
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Registered trials

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.