Evidence map›Paper›PMID 28463821›Full record

ArticleRedox biology2017

Mitochondrial ROS induced by chronic ethanol exposure promote hyper-activation of the NLRP3 inflammasome.

Laura R Hoyt, Matthew J Randall, Jennifer L Ather, Daniel P DePuccio, Christopher C Landry, Xi Qian, Yvonne M Janssen-Heininger, Albert van der Vliet, Anne E Dixon, Eyal Amiel and 1 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Redox biology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 62 papers.

0numbers the graph read from it
0cells of the map it votes in
62citing papers in PubMed
5.5field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

62 citing papers in PubMed, 135 citations in OpenAlex.

  1. Global deletion ofbioRxiv : the preprint server for biology · 2026
    Article
  2. Article
  3. Review
  4. Thiamine Deficiency and Neuroinflammation Are Important Contributors to Alcohol Use Disorder.Pathophysiology : the official journal of the International Society for Pathophysiology · 2025
    Review
  5. Article
  6. Review
  7. Review
  8. Article
  9. Alcohol Consumption and Autoimmune Diseases.International journal of molecular sciences · 2025
    Review
  10. Article
  11. Review
  12. Article
  13. Progress in the Correlation Between Inflammasome NLRP3 and Liver Fibrosis.Journal of clinical and translational hepatology · 2024
    Review
  14. Article
  15. Review
  16. Article
  17. Review
  18. Enhanced CaNature communications · 2023
    Article
  19. Review
  20. Review

2 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 1 institution in 1 country.

Laura R HoytVermont Lung Center, University of Vermont, Burlington, VT 05405, USA; Division of Pulmonary Disease and Critical Care, Department of Medicine, University of Vermont, Burlington, VT 05405, USA.
Matthew J RandallVermont Lung Center, University of Vermont, Burlington, VT 05405, USA; Division of Pulmonary Disease and Critical Care, Department of Medicine, University of Vermont, Burlington, VT 05405, USA.
Jennifer L AtherVermont Lung Center, University of Vermont, Burlington, VT 05405, USA; Division of Pulmonary Disease and Critical Care, Department of Medicine, University of Vermont, Burlington, VT 05405, USA.
Daniel P DePuccioDepartment of Chemistry, University of Vermont, Burlington, VT 05405, USA.
Christopher C LandryDepartment of Chemistry, University of Vermont, Burlington, VT 05405, USA; Cellular, Molecular, and Biomedical Sciences Graduate Program, University of Vermont, Burlington, VT 05405, USA.
Xi QianVermont Lung Center, University of Vermont, Burlington, VT 05405, USA; Department of Pathology and Laboratory Medicine, University of Vermont, Burlington, VT 05405, USA.
Yvonne M Janssen-HeiningerVermont Lung Center, University of Vermont, Burlington, VT 05405, USA; Department of Pathology and Laboratory Medicine, University of Vermont, Burlington, VT 05405, USA; Cellular, Molecular, and Biomedical Sciences Graduate Program, University of Vermont, Burlington, VT 05405, USA.
Albert van der VlietVermont Lung Center, University of Vermont, Burlington, VT 05405, USA; Department of Pathology and Laboratory Medicine, University of Vermont, Burlington, VT 05405, USA; Cellular, Molecular, and Biomedical Sciences Graduate Program, University of Vermont, Burlington, VT 05405, USA.
Anne E DixonVermont Lung Center, University of Vermont, Burlington, VT 05405, USA; Division of Pulmonary Disease and Critical Care, Department of Medicine, University of Vermont, Burlington, VT 05405, USA.
Eyal AmielDepartment of Medical Laboratory and Radiation Sciences, University of Vermont, Burlington, VT 05405, USA; Cellular, Molecular, and Biomedical Sciences Graduate Program, University of Vermont, Burlington, VT 05405, USA.
Matthew E PoynterVermont Lung Center, University of Vermont, Burlington, VT 05405, USA; Division of Pulmonary Disease and Critical Care, Department of Medicine, University of Vermont, Burlington, VT 05405, USA; Cellular, Molecular, and Biomedical Sciences Graduate Program, University of Vermont, Burlington, VT 05405, USA. Electronic address: matthew.poynter@uvm.edu.
University of Vermont · US

Funding

Vermont Immunobioloigy/ Infectious Diseases CenterP20GM103496 · NIGMS · UNIVERSITY OF VERMONT & ST AGRIC COLLEGE · PI BUDD, RALPH C · 2012 to 2015
$8.7M
S-glutathionylation chemistry in fibrotic lung remodelingR35HL135828 · NHLBI · UNIVERSITY OF VERMONT & ST AGRIC COLLEGE · PI JANSSEN-HEININGER, YVONNE M. W. · 2017 to 2023
$6.5M
Multidisciplinary Training in Lung BiologyT32HL076122 · NHLBI · UNIVERSITY OF VERMONT &ST AGRIC COLLEGE · PI IRVIN, CHARLES G, POYNTER, MATTHEW E · 2004 to 2023
$6.1M
Dual Oxidase in Airway Epithelial Repair and RemodelingR01HL085646 · NHLBI · UNIVERSITY OF VERMONT & ST AGRIC COLLEGE · PI VAN DER VLIET, ALBERT · 2008 to 2020
$4.7M
Obese Allergic Asthma and the Impact of Weight Loss on Airway Epithelial FunctionR01HL133920 · NHLBI · UNIVERSITY OF VERMONT & ST AGRIC COLLEGE · PI DIXON, ANNE E, POYNTER, MATTHEW E · 2016 to 2020
$3.9M
Translational Research in Lung Biology and DiseaseP30GM103532 · NIGMS · UNIVERSITY OF VERMONT & ST AGRIC COLLEGE · PI IRVIN, CHARLES G · 2012 to 2016
$3.7M
Non-Allergic Late-Onset Asthma of Obesity: Pathophysiology and TherapyR01HL130847 · NHLBI · UNIVERSITY OF VERMONT & ST AGRIC COLLEGE · PI BATES, JASON HT, DIXON, ANNE E · 2016 to 2019
$2.3M
DUOX1 and Mitochondria in Obese AsthmaR01HL138708 · NHLBI · UNIVERSITY OF VERMONT & ST AGRIC COLLEGE · PI VAN DER VLIET, ALBERT · 2017 to 2020
$2.1M
Serum Amyloid A3 in Allergic Asthma: An Endogenous Mediator of Disease SeverityR01HL107291 · NHLBI · UNIVERSITY OF VERMONT & ST AGRIC COLLEGE · PI POYNTER, MATTHEW E · 2012 to 2015
$1.5M
DUOX1 Silencing in Age-Related COPDR21AG055325 · NIA · UNIVERSITY OF VERMONT & ST AGRIC COLLEGE · PI VAN DER VLIET, ALBERT · 2017 to 2018
$429k
NHLBI NIH HHS R01 HL085646NHLBI NIH HHS R01 HL107291NHLBI NIH HHS R01 HL130847NHLBI NIH HHS R01 HL133920NHLBI NIH HHS R01 HL138708NHLBI NIH HHS R35 HL135828NHLBI NIH HHS T32 HL076122NIA NIH HHS R21 AG055325NIGMS NIH HHS P20 GM103496NIGMS NIH HHS P30 GM103532
6 · The paper itself

Abstract

Alcohol use disorders are common both in the United States and globally, and are associated with a variety of co-morbid, inflammation-linked diseases. The pathogenesis of many of these ailments are driven by the activation of the NLRP3 inflammasome, a multi-protein intracellular pattern recognition receptor complex that facilitates the cleavage and secretion of the pro-inflammatory cytokines IL-1β and IL-18. We hypothesized that protracted exposure of leukocytes to ethanol would amplify inflammasome activation, which would help to implicate mechanisms involved in diseases associated with both alcoholism and aberrant NLRP3 inflammasome activation. Here we show that long-term ethanol exposure of human peripheral blood mononuclear cells and a mouse macrophage cell line (J774) amplifies IL-1β secretion following stimulation with NLRP3 agonists, but not with AIM2 or NLRP1b agonists. The augmented NRLP3 activation was mediated by increases in iNOS expression and NO production, in conjunction with increases in mitochondrial membrane depolarization, oxygen consumption rate, and ROS generation in J774 cells chronically exposed to ethanol (CE cells), effects that could be inhibited by the iNOS inhibitor SEITU, the NO scavenger carboxy-PTIO, and the mitochondrial ROS scavenger MitoQ. Chronic ethanol exposure did not alter K

Indexed as

AnimalsCell LineEthanolGene Expression RegulationHumansInflammasomesInterleukin-1betaLeukocytes, MononuclearMacrophagesMiceMitochondriaNLR Family, Pyrin Domain-Containing 3 ProteinReactive Oxygen SpeciesEthanolInflammasomesInterleukin-1betaNLR Family, Pyrin Domain-Containing 3 ProteinReactive Oxygen SpeciesEthanolIL-1βInflammasomeInflammation

Identifiers

PMID28463821
PMCPMC5413213
OpenAlexW2607833636

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.