ArticleThe journals of gerontology. Series A, Biological sciences and medical sciences2017
Digital PCR Quantitation of Muscle Mitochondrial DNA: Age, Fiber Type, and Mutation-Induced Changes.
Article in The journals of gerontology. Series A, Biological sciences and medical sciences, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 32 citations in OpenAlex.
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- Integrated proteomics and transcriptomics analysis of dynamic changes in muscle fiber types in different regions of porcine skeletal muscle.Advanced biotechnology · 2025Article
- Mitochondrial haplotype and sex modulate responses to endurance exercise training.The Journal of physiology · 2025Article
- Age-induced changes in skeletal muscle mitochondrial DNA synthesis, quantity, and quality in genetically unique rats.GeroScience · 2025Article
- Chimeric mitochondrial RNA transcripts predict mitochondrial genome deletion mutations in mitochondrial genetic diseases and aging.Genome research · 2025Article
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- Temporal expression of mitochondrial life cycle markers during acute and chronic overload of rat plantaris muscles.Frontiers in physiology · 2024Article
- Review
- Nanopore sequencing identifies a higher frequency and expanded spectrum of mitochondrial DNA deletion mutations in human aging.Aging cell · 2023Article
- Age- and time-dependent mitochondrial genotoxic and myopathic effects of beta-guanidinopropionic acid, a creatine analog, on rodent skeletal muscles.GeroScience · 2023Article
- Metformin Treatment in Old Rats and Effects on Mitochondrial Integrity.Rejuvenation research · 2021Article
- Mitochondrial DNA deletion mutations increase exponentially with age in human skeletal muscle.Aging clinical and experimental research · 2021Article
- Skeletal muscle mitochondrial DNA copy number and mitochondrial DNA deletion mutation frequency as predictors of physical performance in older men and women.GeroScience · 2021Article
- Mitochondrial DNA variants in inclusion body myositis characterized by deep sequencing.Brain pathology (Zurich, Switzerland) · 2021Article
- A ketogenic diet impacts markers of mitochondrial mass in a tissue specific manner in aged mice.Aging · 2021Article
- Age-induced mitochondrial DNA point mutations are inadequate to alter metabolic homeostasis in response to nutrient challenge.Aging cell · 2020Article
- Comment on: "Mitochondrial Mechanisms of Neuromuscular Junction Degeneration with Aging.Cells · 2020Article
- Increased burden of mitochondrial DNA deletions and point mutations in early-onset age-related hearing loss in mitochondrial mutator mice.Experimental gerontology · 2019Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
Abstract
Definitive quantitation of mitochondrial DNA (mtDNA) and mtDNA deletion mutation abundances would help clarify the role of mtDNA instability in aging. To more accurately quantify mtDNA, we applied the emerging technique of digital polymerase chain reaction to individual muscle fibers and muscle homogenates from aged rodents. Individual fiber mtDNA content correlated with fiber type and decreased with age. We adapted a digital polymerase chain reaction deletion assay that was accurate in mixing experiments to a mutation frequency of 0.03% and quantitated an age-induced increase in deletion frequency from rat muscle homogenates. Importantly, the deletion frequency measured in muscle homogenates strongly correlated with electron transport chain-deficient fiber abundance determined by histochemical analyses. These data clarify the temporal accumulation of mtDNA deletions that lead to electron chain-deficient fibers, a process culminating in muscle fiber loss.
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Registered trials
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