ArticlePloS one2017
Influence of heat stress, sex and genetic groups on reference genes stability in muscle tissue of chicken.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 36 citations in OpenAlex.
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- Advances in Genomics and Postgenomics in Poultry Science: Current Achievements and Future Directions.International journal of molecular sciences · 2025Review
- Screening of reliable reference genes for the normalization of RT-qPCR in chicken liver tissues and LMH cells.Scientific reports · 2024Article
- Neutral markers reveal complex population structure across the range of a widespread songbird.Ecology and evolution · 2024Article
- Exosome Therapy: A Novel Approach for Enhancing Estrogen Levels in Perimenopause.International journal of molecular sciences · 2024Article
- Selection of reference genes for RT-qPCR analysis in developing chicken embryonic ovary.Molecular biology reports · 2023Article
- Evaluation of Catechin Synergistic and Antibacterial Efficacy on Biofilm Formation andAntibiotics (Basel, Switzerland) · 2022Article
- Article
- Investigation of chicken housekeeping genes using next-generation sequencing data.Frontiers in genetics · 2022Article
- Comparison of reference gene expression stability in mouse skeletal muscle via five algorithms.PeerJ · 2022Article
- Screening of Reference Genes for RT-qPCR in Chicken Adipose Tissue and Adipocytes.Frontiers in physiology · 2021Article
- Differential Expression of Myogenic and Calcium Signaling-Related Genes in Broilers Affected With White Striping.Frontiers in physiology · 2021Article
- Selection of reference genes for quantitative real-time PCR normalization in European quail tissues.Molecular biology reports · 2021Article
- Heat shock protein B1 expression is associated with age at sexual maturity in Rhode Island Red and Punjab Red layers under heat stress.International journal of biometeorology · 2020Article
- Selection and Validation of the Optimal Panel of Reference Genes for RT-qPCR Analysis in the Developing Rat Cartilage.Frontiers in genetics · 2020Article
- Protective Effects of 1,8-Cineole Microcapsules Against Inflammation and Gut Microbiota Imbalance Associated Weight Loss Induced by Heat Stress in Broiler Chicken.Frontiers in pharmacology · 2020Article
- Reference genes for proximal femoral epiphysiolysis expression studies in broilers cartilage.PloS one · 2020Article
- Influence of heat stress on reference genes stability in heart and liver of two chickens genotypes.PloS one · 2020Article
- Stable reference genes for expression studies in breast muscle of normal and white striping-affected chickens.Molecular biology reports · 2020Article
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Quantitative RT-PCR is an important technique for assessing gene expression. However, a proper normalization of reference genes prior to expression analyses of target genes is necessary. The best normalizer is that gene which remains stable in all samples from different treatments. The aim of this study was to identify stable reference genes for normalization of target genes in muscle tissue from three genetically divergent chickens groups (Peloco, Cobb 500® and Caneluda) under environmental (heat stress and comfort) and sex influence. Expressions of ten reference genes were tested for stability in breast muscular tissue (Pectoralis major muscle). Samples were obtained from 36 males and females of two backyard breeds (Caneluda and Peloco) and one commercial line (Cobb 500®) under two environments. The heat stress and comfort temperature were 39 and 23°C, respectively. Animals were housed in the Animal Science Department at Universidade Estadual do Sudoeste da Bahia. We analyzed the expression data by four statistical tools (SLqPCR, NormFinder, Bestkeeper and Comparative CT). According to these tools, genes stability varied according to sex, genetic group and environment, however, some genes remained stable in all analyzes. There was no difference between the most stable genes for sex effect, being MRPS27 more stable for both males and females. In general, MRPS27 was the most stable gene. Within the three genetic groups, the most stable genes were RPL5, HMBS and EEF1 to Cobb 500®, Peloco and Caneluda, respectively. Within the environment, the most stable gene under comfort and heat stress conditions was HMBS and MRPS27, respectively. BestKeeper and Comparative Ct were less correlated (28%) and SLqPCR and NormFinder were the most correlated (98%). MRPS27, RPL5 and MRPS30 genes were considered stable according the overall ranking and can be used as normalizer of relative expression of target genes in muscle tissue of chickens under heat stress.
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