ArticleThe Biochemical journal1988
Protein phosphorylation in electrically permeabilized islets of Langerhans. Effects of Ca2+, cyclic AMP, a phorbol ester and noradrenaline.
Article in The Biochemical journal, 1988. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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Who cites it
48 citing papers in PubMed, 155 citations in OpenAlex.
- NAMPT activity plays a key role in driving autoimmune processes that mediate beta-cell death and type 1 diabetes development in mice.Cell death & disease · 2026Article
- Mesenchymal stromal cell secretory molecules improve the functional survival of human islets.Diabetic medicine : a journal of the British Diabetic Association · 2023Article
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- Drug reformulation for a neglected disease. The NANOHAT project to develop a safer more effective sleeping sickness drug.PLoS neglected tropical diseases · 2021Article
- The cannabinoid ligands SR141716A and AM251 enhance human and mouse islet function via GPR55-independent signalling.Cellular and molecular life sciences : CMLS · 2020Article
- Psoriatic skin inflammation induces a pre-diabetic phenotype via the endocrine actions of skin secretome.Molecular metabolism · 2020Article
- UCN2: a new candidate influencing pancreatic β-cell adaptations in pregnancy.The Journal of endocrinology · 2020Article
- Optimizing beta cell function through mesenchymal stromal cell-mediated mitochondria transfer.Stem cells (Dayton, Ohio) · 2020Article
- Structure-functional changes in eNAMPT at high concentrations mediate mouse and human beta cell dysfunction in type 2 diabetes.Diabetologia · 2020Article
- Characterization of the Effects of Mesenchymal Stromal Cells on Mouse and Human Islet Function.Stem cells translational medicine · 2019Article
- Wingless-Type MMTV Integration Site Family Member 5a Is a Key Secreted Islet Stellate Cell-Derived Product that Regulates Islet Function.International journal of endocrinology · 2019Article
- The adhesion receptor GPR56 is activated by extracellular matrix collagen III to improve β-cell function.Cellular and molecular life sciences : CMLS · 2018Article
- Exendin‑4 reverses endothelial dysfunction in mice fed a high‑cholesterol diet by a GTP cyclohydrolase‑1/tetrahydrobiopterin pathway.Molecular medicine reports · 2018Article
- Intracellular Delivery by Membrane Disruption: Mechanisms, Strategies, and Concepts.Chemical reviews · 2018Review
- Defining G protein-coupled receptor peptide ligand expressomes and signalomes in human and mouse islets.Cellular and molecular life sciences : CMLS · 2018Article
- C3aR and C5aR1 act as key regulators of human and mouse β-cell function.Cellular and molecular life sciences : CMLS · 2018Article
- Regulation of insulin exocytosis by calcium-dependent protein kinase C in beta cells.Cell calcium · 2017Review
- Carnosine scavenging of glucolipotoxic free radicals enhances insulin secretion and glucose uptake.Scientific reports · 2017Article
- Polycystic Kidney Disease with Hyperinsulinemic Hypoglycemia Caused by a Promoter Mutation in Phosphomannomutase 2.Journal of the American Society of Nephrology : JASN · 2017Article
- A comparative analysis of human and mouse islet G-protein coupled receptor expression.Scientific reports · 2017Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The incorporation of 32P from [gamma-32P]ATP into intracellular proteins was studied in electrically permeabilized rat islets of Langerhans. Ca2+ (10 microM), cyclic AMP (100 microM) and a protein kinase C-activating phorbol ester, phorbol 13-myristate 12-acetate (PMA; 100 nM) produced marked changes in the phosphorylation state of a number of proteins in permeabilized islets after incubation for 1 min at 37 degrees C. Ca2+ modified the effects of cyclic AMP and PMA on protein phosphorylation. Noradrenaline (10 microM) had no detectable effects on Ca2+-dependent protein phosphorylation, but significantly inhibited Ca2+-induced insulin secretion from electrically permeabilized islets. These results suggest that electrically permeabilized islets offer a useful model in which to study rapid events in protein phosphorylation as a mechanism of stimulus-secretion coupling. If the rapid Ca2+-induced effects on protein phosphorylation are involved in the control of insulin secretion, the results of this study also imply that part of the catecholamine inhibition of insulin secretion occurs at a stage in the secretory pathway beyond the activation of the regulated protein kinases.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.