ArticleJournal of physics D: Applied physics2017
Dissecting the actin cortex density and membrane-cortex distance in living cells by super-resolution microscopy.
Article in Journal of physics D: Applied physics, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
33 citing papers in PubMed.
- The membrane-to-cortex distance regulates mDia1 activity to control cortical mechanics.Nature communications · 2026Article
- Bacterial Outer Membrane Vesicles: Research Advances from Biogenesis Mechanisms to Engineered Applications.Membranes · 2026Review
- Clustering hinders APP α-secretase processing in the plasma membrane.Biophysical journal · 2026Article
- A single-molecule reporter of membrane-proximal actin detects rapid remodeling upon B cell receptor clustering.bioRxiv : the preprint server for biology · 2026Article
- Ghosts on the Membrane: Cytoskeletal Pinning Influences Nanoscale Cell Membrane Organization.Biomolecules · 2026Article
- Sub-membrane actin rings compartmentalize the plasma membrane.The Journal of cell biology · 2024Article
- T cell migration requires ion and water influx to regulate actin polymerization.Nature communications · 2023Article
- Crk proteins activate the Rap1 guanine nucleotide exchange factor C3G by segregated adaptor-dependent and -independent mechanisms.Cell communication and signaling : CCS · 2023Article
- Quantifying cytoskeletal organization from optical microscopy data.Frontiers in cell and developmental biology · 2023Review
- Actomyosin-Driven Division of a Synthetic Cell.ACS synthetic biology · 2022Review
- Structural mechanism for bidirectional actin cross-linking by T-plastin.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Heterogeneous nanoscopic lipid diffusion in the live cell membrane and its dependency on cholesterol.Biophysical journal · 2022Article
- Actin nano-architecture of phagocytic podosomes.Nature communications · 2022Article
- Simple methods for quantifying super-resolved cortical actin.Scientific reports · 2022Article
- Article
- iASPP contributes to cell cortex rigidity, mitotic cell rounding, and spindle positioning.The Journal of cell biology · 2021Article
- Actomyosin dynamics modulate microtubule deformation and growth during T-cell activation.Molecular biology of the cell · 2021Article
- Comparative mapping of crawling-cell morphodynamics in deep learning-based feature space.PLoS computational biology · 2021Article
- Pinching the cortex of live cells reveals thickness instabilities caused by myosin II motors.Science advances · 2021Article
- Super-Resolution Imaging Approaches for Quantifying F-Actin in Immune Cells.Frontiers in cell and developmental biology · 2021Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
Abstract
Nanoscale spacing between the plasma membrane and the underlying cortical actin cytoskeleton profoundly modulates cellular morphology, mechanics, and function. Measuring this distance has been a key challenge in cell biology. Current methods for dissecting the nanoscale spacing either limit themselves to complex survey design using fixed samples or rely on diffraction-limited fluorescence imaging whose spatial resolution is insufficient to quantify distances on the nanoscale. Using dual-color super-resolution STED (stimulated-emission-depletion) microscopy, we here overcome this challenge and accurately measure the density distribution of the cortical actin cytoskeleton and the distance between the actin cortex and the membrane in live Jurkat T-cells. We found an asymmetric cortical actin density distribution with a mean width of 230 (+105/-125) nm. The spatial distances measured between the maximum density peaks of the cortex and the membrane were bi-modally distributed with mean values of 50 ± 15 nm and 120 ± 40 nm, respectively. Taken together with the finite width of the cortex, our results suggest that in some regions the cortical actin is closer than 10 nm to the membrane and a maximum of 20 nm in others.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.