ArticleJournal of assisted reproduction and genetics2017
Is early embryo development as observed by time-lapse microscopy dependent on whether fresh or frozen sperm was used for ICSI? A cohort study.
Article in Journal of assisted reproduction and genetics, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
13 citing papers in PubMed, 19 citations in OpenAlex.
- Influence of seminal cryopreservation on embryo kinetics. Image analysis and clinical outcomes.JBRA assisted reproduction · 2026Article
- The Influence of Cryopreservation on Sperm Morphology and Its Implications in Terms of Fractions of Higher-Quality Sperm.Journal of clinical medicine · 2024Article
- Effect of freezing and thawing on ejaculated sperm and subsequent pregnancy and neonatal outcomes in IVF.Frontiers in endocrinology · 2024Article
- Influence of a hyaluronan-binding system for sperm selection in intracytoplasmic sperm injection cycles on embryo morphokinetic parameters and in vitro fertilization cycle outcomes.Archives of gynecology and obstetrics · 2023Article
- What Does Intracytoplasmic Sperm Injection Change in Embryonic Development? The Spermatozoon Contribution.Journal of clinical medicine · 2023Review
- Poultry genetic heritage cryopreservation and reconstruction: advancement and future challenges.Journal of animal science and biotechnology · 2022Review
- Outcomes of Frozen Oocyte Donor In Vitro Fertilization (IVF) Cycles Using Fresh Versus Frozen Sperm.Reproductive sciences (Thousand Oaks, Calif.) · 2022Article
- Male Age and Progressive Sperm Motility Are Critical Factors Affecting Embryological and Clinical Outcomes in Oocyte Donor ICSI Cycles.Reproductive sciences (Thousand Oaks, Calif.) · 2022Article
- The presence of cytoplasmic strings in human blastocysts is associated with the probability of clinical pregnancy with fetal heart.Journal of assisted reproduction and genetics · 2021Article
- Time-lapse imaging of human embryos fertilized with testicular sperm reveals an impact on the first embryonic cell cycle.Biology of reproduction · 2021Article
- The outcome of IVF/ICSI cycles in male cancer patients: retrospective analysis of procedures from 2004 to 2018.Radiology and oncology · 2021Article
- Good practice recommendations for the use of time-lapse technologyHuman reproduction open · 2020Article
- Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeThe aim of this study was to compare timings of key events of embryo development from those originating from either fresh or cryopreserved ejaculate sperm using time-lapse technology.
methodsIn this retrospective observational cohort study, time-lapse technology was used to monitor 1927 embryos from 234 women undergoing intracytoplasmic sperm injection (ICSI) and utilizing either fresh (n = 172 cycles) or cryopreserved ejaculate sperm (n = 62 cycles) for insemination were included in the study. Key developmental events as described in time-lapse were compared with the use of generalized estimating equations (GEE) to adjust for any auto-correlation between the observations. In addition, multivariable logit regression models were used to account for any known baseline differences between the two groups.
resultsThere were no differences in conventional embryo development such as number of 8-cell embryos by 72 h (p = 0.359), the number of blastocysts by 120 h (p = 0.417), and the number of top quality blastocysts (p = 0.956) between the two groups compared. There were no statistical differences in the timings of any of the key embryo developmental events (PN_t1, NEBD, cytokinesis, t2, t3, t4, t5, t6, t7, t8, tM, tSB, tEB, tHB, s1, s2, s3, cc2, and cc3) when either fresh or cryopreserved ejaculate sperm was used for ICSI. This was also confirmed with conventional morphological assessment.
conclusionsThis observational cohort study has shown that there are no differences in the morphokinetic parameters of early embryo development when either fresh or frozen ejaculate sperm are used for ICSI insemination.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.