Evidence map›Paper›PMID 28453955›Full record

ArticleBiomaterials2017

Acoustic tweezing cytometry enhances osteogenesis of human mesenchymal stem cells through cytoskeletal contractility and YAP activation.

Xufeng Xue, Xiaowei Hong, Zida Li, Cheri X Deng, Jianping Fu

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomaterials, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.

0numbers the graph read from it
0cells of the map it votes in
41citing papers in PubMed
2.9field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

41 citing papers in PubMed, 80 citations in OpenAlex.

  1. Article
  2. Review
  3. Deep learning reveals how cells pull, buckle, and navigate fibrous environments.Proceedings of the National Academy of Sciences of the United States of America · 2025
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  18. Nuclear compression regulates YAP spatiotemporal fluctuations in living cells.Proceedings of the National Academy of Sciences of the United States of America · 2023
    Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Xufeng XueDepartment of Mechanical Engineering, University of Michigan, Ann Arbor, MI 48109, USA.
Xiaowei HongDepartment of Biomedical Engineering, University of Michigan, Ann Arbor, MI 48109, USA.
Zida LiDepartment of Mechanical Engineering, University of Michigan, Ann Arbor, MI 48109, USA.
Cheri X DengDepartment of Mechanical Engineering, University of Michigan, Ann Arbor, MI 48109, USA; Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI 48109, USA. Electronic address: cxdeng@umich.edu.
Jianping FuDepartment of Mechanical Engineering, University of Michigan, Ann Arbor, MI 48109, USA; Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI 48109, USA; Department of Cell and Developmental Biology, University of Michigan Medical School, Ann Arbor, MI 48109, USA. Electronic address: jpfu@umich.edu.
University of Michigan–Ann Arbor · US

Funding

Acoustic tweezing cytometry: technology development and stem cell applicationsR01EB019436 · NIBIB · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI DENG, CHERI X, FU, JIANPING · 2015 to 2018
$1.9M
Ultrasound-mediated Directed Osteogenic Differentiation of Mesenchymal Stem CellsR21EB017078 · NIBIB · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI DENG, CHERI X, FU, JIANPING · 2014 to 2015
$428k
NIBIB NIH HHS R01 EB019436NIBIB NIH HHS R21 EB017078
6 · The paper itself

Abstract

Human mesenchymal stem cells (hMSCs) have great potential for cell-based therapies for treating degenerative bone diseases. It is known that mechanical cues in the cell microenvironment play an important role in regulating osteogenic (bone) differentiation of hMSCs. However, mechanoregulation of lineage commitment of hMSCs in conventional two-dimensional (2D) monocultures or bioengineered three-dimensional (3D) tissue constructs remains suboptimal due to complex biomaterial design criteria for hMSC culture. In this study, we demonstrate the feasibility of a novel cell mechanics and mechanobiology tool, acoustic tweezing cytometry (ATC), for mechanical stimulation of hMSCs. ATC utilizes ultrasound (US) pulses to actuate functionalized lipid microbubbles (MBs) which are covalently attached to hMSCs via integrin binding to exert forces to the cells. ATC stimulation increases cytoskeletal contractility of hMSCs regardless of the cell area. Furthermore, ATC application rescues osteogenic differentiation of hMSCs in culture conditions that are intrinsically repressive for hMSC osteogenesis (e.g., soft cell culture surfaces). ATC application activates transcriptional regulator YAP to enhance hMSC osteogenesis. Our data further show that F-actin, myosin II, and RhoA/ROCK signaling functions upstream of YAP activity in mediating ATC-stimulated hMSC osteogenesis. With the capability of applying controlled dynamic mechanical stimuli to cells, ATC provides a powerful tool for mechanoregulation of stem cell behaviors in tissue engineering and regenerative medicine applications.

Indexed as

Blotting, WesternCell DifferentiationCells, CulturedCytological TechniquesCytoskeletonHumansMesenchymal Stem CellsMicrobubblesOsteogenesisReal-Time Polymerase Chain ReactionSignal TransductionAcoustic tweezing cytometryHuman mesenchymal stem cellsMechanotransductionOsteogenesisYAP signaling

Identifiers

PMID28453955
PMCPMC5506541
OpenAlexW2605496274

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.