ArticlePloS one2017
LC-MS-MS quantitative analysis reveals the association between FTO and DNA methylation.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
16 citing papers in PubMed, 26 citations in OpenAlex.
- Evidence human FTO catalyses hydroxylation of N6-methyladenosine without direct formation of a demethylated product contrasting with ALKBH5/2/3 and bacterial AlkB.Nucleic acids research · 2025Article
- Mitochondrial transcriptome of Candida albicans in flagranti - direct RNA sequencing reveals a new layer of information.BMC genomics · 2024Article
- Review
- The landscape of implantation and placentation: deciphering the function of dynamic RNA methylation at the maternal-fetal interface.Frontiers in endocrinology · 2023Review
- The multifaceted functions of the Fat mass and Obesity-associated protein (FTO) in normal and cancer cells.RNA biology · 2022Article
- Broad-range RNA modification analysis of complex biological samples using rapid C18-UPLC-MS.RNA biology · 2021Article
- UPLC-MS/MS method for simultaneously determining nucleosides and methyl-nucleosides in liver mRNA of Epimedin C-induced liver injury mouse model.Chinese medicine · 2021Article
- The dynamics of NGenome biology · 2021Article
- Demethyltransferase AlkBH1 substrate diversity and relationship to human diseases.Molecular biology reports · 2021Review
- NADP modulates RNA mNature chemical biology · 2020Article
- m6A RNA Methylation: Ramifications for Gene Expression and Human Health.Molecular biotechnology · 2020Review
- A molecular-level perspective on the frequency, distribution, and consequences of messenger RNA modifications.Wiley interdisciplinary reviews. RNA · 2020Review
- Pan-cancer analysis reveals synergistic effects of CDK4/6i and PARPi combination treatment in RB-proficient and RB-deficient breast cancer cells.Cell death & disease · 2020Article
- FTO haplotyping underlines high obesity risk for European populations.BMC medical genomics · 2019Article
- RNA-modifying proteins as anticancer drug targets.Nature reviews. Drug discovery · 2018Review
- New Insight into the Octamer of TYMS Stabilized by Intermolecular Cys43-Disulfide.International journal of molecular sciences · 2018Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fat mass and obesity-associated protein (FTO) is α-ketoglutarate-dependent dioxygenase and responsible for demethylating N6-methyladenosine (m6A) in mRNA, 3-methylthymine (m3T) in single-stranded DNA (ssDNA) and 3-methyluracil (m3U) in single-stranded RNA (ssRNA). Its other function remains unknown but thousands of mammalian DNA show 5-methyl-2'-deoxycytidine (5mdC) modification and 5mdC demethylases are required for mammalian energy homeostasis and fertility. Here, we aimed to confirm whether FTO proteins can demethylate 5mdC in DNA. However, we found that FTO exhibits no potent demethylation activity against 5mdC in vitro and in vivo by using liquid chromatography-tandem mass spectrometry (LC-MS-MS). The result showed FTO demethylase has the characteristics of high substrates specificity and selectivity. In addition, we also used immunofluorescence technique to demonstrate overexpression of wild type TET2, but not FTO and mutant TET2 in Hela cells results in higher levels of 5-hydroxymethyl-2'-deoxycytidine (5hmdC) generated from 5mdC. In conclusion, our results not only reveal the enzymatic activity of FTO, but also may facilitate the future discovery of proteins involved in epigenetic modification function.
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